Fenofibrate protects the intestine against ischemia/reperfusion injury

Carlos Rodrigo Camara-Lemarroy, Francisco J. Guzman-de La Garza, Paula Cordero-Perez, Gabriela Alarcon-Galvan, Liliana Torres-Gonzalez, Linda E. Munoz-Espinosa, Nancy E. Fernandez-Garza

Research output: Contribution to journalArticle

Abstract

Background: Ischemia/reperfusion (I/R) injury is a potentially devastating condition, associated with a systemic inflammatory response. It occurs during shock, transplant procedures, or vascular surgery. Objective: We evaluated the protective effects of Fenofibrate (FEN) over intestinal I/R injury. Materials and methods: Intestinal I/R was induced in male Wistar rats by clamping the superior mesenteric artery for 60 minutes, followed by 60 minutes of reperfusion. Rats either received saline or FEN (100 mg/k, via gavage) daily, for three days before inducing I/R. Sham operated rats were used as normal controls. At the end of the procedure, tissue and blood samples were obtained. Serum concentrations of AST, ALT, LDH, tumor necrosis factor-alpha (TNF-alpha), malonaldehyde (MDA), and total antioxidant capacity (TAC) were determined. A histopathological analysis was also performed. Results: After I/R, there was evident tissue injury, as well as serum elevations of AST, ALT, and LDH concentrations. These alterations were reduced by FEN treatment. TNF-alpha concentrations were increased in saline treated animals when compared with FEN treated group (2.26±1 ng/ml vs. 0.23±0.41 ng/ml, respectively, p <0.05). A similar pattern was observed in MDA levels (7.42±1.72 μM/ml vs. 1.72±0.61 μM/ml, respectively, p <0.05). TAC was reduced in saline treated animals (2.05±0.36 Trolox-Equivalents), but preserved in the FEN treated group (3.08±0.36 Trolox-Equivalents, p <0.05). Conclusion: FEN reduced intestinal I/R injury, probably due to anti-inflammatory and antioxidant properties. Its usefulness as a treatment for I/R should be studied.
Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalAsian Biomedicine
DOIs
Publication statusPublished - 1 Apr 2012
Externally publishedYes

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Fenofibrate
Reperfusion Injury
Intestines
Reperfusion
Rats
Ischemia
Antioxidants
Animals
Tumor Necrosis Factor-alpha
Tissue
Transplants
Superior Mesenteric Artery
Malondialdehyde
Serum
Constriction
Surgery
Blood Vessels
Wistar Rats
Shock
Blood

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Camara-Lemarroy, C. R., Guzman-de La Garza, F. J., Cordero-Perez, P., Alarcon-Galvan, G., Torres-Gonzalez, L., Munoz-Espinosa, L. E., & Fernandez-Garza, N. E. (2012). Fenofibrate protects the intestine against ischemia/reperfusion injury. Asian Biomedicine, 279-283. https://doi.org/10.5372/1905-7415.0602.054
Camara-Lemarroy, Carlos Rodrigo ; Guzman-de La Garza, Francisco J. ; Cordero-Perez, Paula ; Alarcon-Galvan, Gabriela ; Torres-Gonzalez, Liliana ; Munoz-Espinosa, Linda E. ; Fernandez-Garza, Nancy E. / Fenofibrate protects the intestine against ischemia/reperfusion injury. In: Asian Biomedicine. 2012 ; pp. 279-283.
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Camara-Lemarroy, CR, Guzman-de La Garza, FJ, Cordero-Perez, P, Alarcon-Galvan, G, Torres-Gonzalez, L, Munoz-Espinosa, LE & Fernandez-Garza, NE 2012, 'Fenofibrate protects the intestine against ischemia/reperfusion injury', Asian Biomedicine, pp. 279-283. https://doi.org/10.5372/1905-7415.0602.054

Fenofibrate protects the intestine against ischemia/reperfusion injury. / Camara-Lemarroy, Carlos Rodrigo; Guzman-de La Garza, Francisco J.; Cordero-Perez, Paula; Alarcon-Galvan, Gabriela; Torres-Gonzalez, Liliana; Munoz-Espinosa, Linda E.; Fernandez-Garza, Nancy E.

In: Asian Biomedicine, 01.04.2012, p. 279-283.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fenofibrate protects the intestine against ischemia/reperfusion injury

AU - Camara-Lemarroy, Carlos Rodrigo

AU - Guzman-de La Garza, Francisco J.

AU - Cordero-Perez, Paula

AU - Alarcon-Galvan, Gabriela

AU - Torres-Gonzalez, Liliana

AU - Munoz-Espinosa, Linda E.

AU - Fernandez-Garza, Nancy E.

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Background: Ischemia/reperfusion (I/R) injury is a potentially devastating condition, associated with a systemic inflammatory response. It occurs during shock, transplant procedures, or vascular surgery. Objective: We evaluated the protective effects of Fenofibrate (FEN) over intestinal I/R injury. Materials and methods: Intestinal I/R was induced in male Wistar rats by clamping the superior mesenteric artery for 60 minutes, followed by 60 minutes of reperfusion. Rats either received saline or FEN (100 mg/k, via gavage) daily, for three days before inducing I/R. Sham operated rats were used as normal controls. At the end of the procedure, tissue and blood samples were obtained. Serum concentrations of AST, ALT, LDH, tumor necrosis factor-alpha (TNF-alpha), malonaldehyde (MDA), and total antioxidant capacity (TAC) were determined. A histopathological analysis was also performed. Results: After I/R, there was evident tissue injury, as well as serum elevations of AST, ALT, and LDH concentrations. These alterations were reduced by FEN treatment. TNF-alpha concentrations were increased in saline treated animals when compared with FEN treated group (2.26±1 ng/ml vs. 0.23±0.41 ng/ml, respectively, p <0.05). A similar pattern was observed in MDA levels (7.42±1.72 μM/ml vs. 1.72±0.61 μM/ml, respectively, p <0.05). TAC was reduced in saline treated animals (2.05±0.36 Trolox-Equivalents), but preserved in the FEN treated group (3.08±0.36 Trolox-Equivalents, p <0.05). Conclusion: FEN reduced intestinal I/R injury, probably due to anti-inflammatory and antioxidant properties. Its usefulness as a treatment for I/R should be studied.

AB - Background: Ischemia/reperfusion (I/R) injury is a potentially devastating condition, associated with a systemic inflammatory response. It occurs during shock, transplant procedures, or vascular surgery. Objective: We evaluated the protective effects of Fenofibrate (FEN) over intestinal I/R injury. Materials and methods: Intestinal I/R was induced in male Wistar rats by clamping the superior mesenteric artery for 60 minutes, followed by 60 minutes of reperfusion. Rats either received saline or FEN (100 mg/k, via gavage) daily, for three days before inducing I/R. Sham operated rats were used as normal controls. At the end of the procedure, tissue and blood samples were obtained. Serum concentrations of AST, ALT, LDH, tumor necrosis factor-alpha (TNF-alpha), malonaldehyde (MDA), and total antioxidant capacity (TAC) were determined. A histopathological analysis was also performed. Results: After I/R, there was evident tissue injury, as well as serum elevations of AST, ALT, and LDH concentrations. These alterations were reduced by FEN treatment. TNF-alpha concentrations were increased in saline treated animals when compared with FEN treated group (2.26±1 ng/ml vs. 0.23±0.41 ng/ml, respectively, p <0.05). A similar pattern was observed in MDA levels (7.42±1.72 μM/ml vs. 1.72±0.61 μM/ml, respectively, p <0.05). TAC was reduced in saline treated animals (2.05±0.36 Trolox-Equivalents), but preserved in the FEN treated group (3.08±0.36 Trolox-Equivalents, p <0.05). Conclusion: FEN reduced intestinal I/R injury, probably due to anti-inflammatory and antioxidant properties. Its usefulness as a treatment for I/R should be studied.

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JO - Asian Biomedicine

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Camara-Lemarroy CR, Guzman-de La Garza FJ, Cordero-Perez P, Alarcon-Galvan G, Torres-Gonzalez L, Munoz-Espinosa LE et al. Fenofibrate protects the intestine against ischemia/reperfusion injury. Asian Biomedicine. 2012 Apr 1;279-283. https://doi.org/10.5372/1905-7415.0602.054