Expression of nerve growth factor in human pancreatic β cells

Román Vidaltamayo, Carlos M. Mery, Arturo Ángeles-Ángeles, Guillermo Robles-Díaz, Marcia Hiriart

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Nerve growth factor (NGF) is an important modulator of rat pancreatic β-cell physiology in vitro. In this study, we analysed the expression of NGF, TrkA and insulin in human pancreatic islets from normal, ductal adenocarcinoma and insulinoma-afflicted samples, using double immunofluorescent labelling and confocal microscopy. We found that in normal human pancreas, insulin and NGF are co-expressed in β cells. Moreover, similar to previous observations in rat, the high affinity NGF receptor TrkA is also expressed in β cells. Pancreatic β cells in normal islets from adenocarcinoma and mucinous cystadenocarcinoma patients also expressed NGF. In 2 out of 15 exocrine tumour samples, NGF was detected also in the tissue surrounding the islets, while 2 out of 13 adenocarcinoma tumours expressed this growth factor. In five insulinoma samples, we observed weaker immunofluorescent labelling of insulin and NGF in the neoplastic tissue, compared to the islets not afflicted by the tumour, which may be a consequence of increased hormone secretion rate. We demonstrate that human β cells express TrkA and NGF. These findings are consistent with the hypothesis that NGF modulates insulin secretion through a paracrine/autocrine loop, similar to the one observed in cultured rat β cells.
Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalGrowth Factors
DOIs
Publication statusPublished - 1 Sep 2003
Externally publishedYes

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Nerve Growth Factor
Insulin
Rats
Tumors
Insulinoma
Adenocarcinoma
Labeling
Mucinous Cystadenocarcinoma
Tissue
Nerve Growth Factor Receptor
Cell Physiological Phenomena
Neoplasms
Confocal microscopy
Physiology
Islets of Langerhans
Confocal Microscopy
Modulators
Pancreas
Cultured Cells
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Vidaltamayo, R., Mery, C. M., Ángeles-Ángeles, A., Robles-Díaz, G., & Hiriart, M. (2003). Expression of nerve growth factor in human pancreatic β cells. Growth Factors, 103-107. https://doi.org/10.1080/08977190310001629566
Vidaltamayo, Román ; Mery, Carlos M. ; Ángeles-Ángeles, Arturo ; Robles-Díaz, Guillermo ; Hiriart, Marcia. / Expression of nerve growth factor in human pancreatic β cells. In: Growth Factors. 2003 ; pp. 103-107.
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Expression of nerve growth factor in human pancreatic β cells. / Vidaltamayo, Román; Mery, Carlos M.; Ángeles-Ángeles, Arturo; Robles-Díaz, Guillermo; Hiriart, Marcia.

In: Growth Factors, 01.09.2003, p. 103-107.

Research output: Contribution to journalArticle

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