Eugenol, citral, and hexanal, alone or in combination with heat, affect viability, biofilm formation, and swarming on Shiga-toxin-producing Escherichia coli.

Cindy Joanna Caballero Prado, José Ángel Merino Mascorro, Norma Heredia, Jorge Dávila Aviña, Santos García*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Shiga-toxin-producing Escherichia coli strains are pathogenic for humans and cause mild to severe illnesses. In this study, the antimicrobial effect of citral, eugenol, and hexanal in combination with heat shock (HS) was evaluated in terms of the growth, biofilm formation, swarming, and expression of virulence genes of STEC serotypes (O157:H7, O103, O111, and O26). Eugenol was the most effective compound against the growth of E. coli strains (MBC = 0.58 to 0.73 mg/mL), followed by citral (MBC = 0.86 to 1.26 mg/mL) and hexanal (MBC = 2.24 to 2.52 mg/mL). Biofilm formation and swarming motility have great variability between STEC strains. Natural compounds—alone or combined with HS—inhibited biofilm formation; however, swarming motility was induced by most treatments. The expression of the studied genes during biofilm formation and swarming under natural antimicrobials was affected but not in a uniform pattern. These treatments could be used to control contamination of STEC and inhibit biofilm formation.
Original languageEnglish
Pages (from-to)599–607
Number of pages9
JournalFood Science and Biotechnology
Volume30
Issue number4
DOIs
Publication statusPublished - 9 Apr 2021
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the Consejo Nacional de Ciencia y Tecnología de Mexico (CONACYT) [285151]. We are also thankful to CONACYT for the scholarship granted to Cindy Joanna Caballero-Prado.

Funding Information:
This study was supported by the Consejo Nacional de Ciencia y Tecnología de Mexico (CONACYT) [285151]. We are also thankful to CONACYT for the scholarship granted to Cindy Joanna Caballero-Prado.

Publisher Copyright:
© 2021, The Korean Society of Food Science and Technology.

All Science Journal Classification (ASJC) codes

  • General Agricultural and Biological Sciences
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology

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