Entrainment of breast cell lines results in rhythmic fluctuations of microRNAs

Rafael Chacolla-Huaringa, Jorge Moreno-Cuevas, Victor Trevino, Sean Patrick Scott

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13 Citations (Scopus)

Abstract

Circadian rhythms are essential for temporal (~24 h) regulation of molecular processes in diverse species. Dysregulation of circadian gene expression has been implicated in the pathogenesis of various disorders, including hypertension, diabetes, depression, and cancer. Recently, microRNAs (miRNAs) have been identified as critical modulators of gene expression post-transcriptionally, and perhaps involved in circadian clock architecture or their output functions. The aim of the present study is to explore the temporal expression of miRNAs among entrained breast cell lines. For this purpose, we evaluated the temporal (28 h) expression of 2006 miRNAs in MCF-10A, MCF-7, and MDA-MB-231 cells using microarrays after serum shock entrainment. We noted hundreds of miRNAs that exhibit rhythmic fluctuations in each breast cell line, and some of them across two or three cell lines. Afterwards, we validated the rhythmic profiles exhibited by miR-141-5p, miR-1225-5p, miR-17-5p, miR-222-5p, miR-769-3p, and miR-548ay-3p in the above cell lines, as well as in ZR-7530 and HCC-1954 using RT-qPCR. Our results show that serum shock entrainment in breast cells lines induces rhythmic fluctuations of distinct sets of miRNAs, which have the potential to be related to endogenous circadian clock, but extensive investigation is required to elucidate that connection.

Original languageEnglish
Article number1499
JournalInternational Journal of Molecular Sciences
Volume18
Issue number7
DOIs
Publication statusPublished - 12 Jul 2017
Externally publishedYes

Bibliographical note

Funding Information:
The authors would like to thank Ana Cristina González and Raul Cantú for their technical support with RNA purification. Rafael Chacolla-Huaringa expresses his gratitude to the Mexican National Council for Science and Technology (CONACyT) for the Ph.D. grant scholarship (#290649). This work was funded by Cátedras de Hematologia y Cancer and Terapia Celular y Medicina Regenerativa, part of the Tecnológico de Monterrey, Monterrey campus.

Publisher Copyright:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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