Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico

J L Gutiérrez-Ferman, L Villarreal-Treviño, J M Ramírez-Aranda, A Camacho-Ortiz, M R Ballesteros-Elizondo, M R Moreno-Juárez, S Mendoza-Olazarán, M E de la O Cavazos, J Z Villarreal-Pérez, M A Gómez-Govea, E Garza-González

Research output: Contribution to journalArticle

Abstract

We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

Original languageEnglish
Pages (from-to)2096-2101
Number of pages6
JournalEpidemiology and Infection
Volume146
Issue number16
DOIs
Publication statusPublished - Dec 2018
Externally publishedYes

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    Gutiérrez-Ferman, J. L., Villarreal-Treviño, L., Ramírez-Aranda, J. M., Camacho-Ortiz, A., Ballesteros-Elizondo, M. R., Moreno-Juárez, M. R., Mendoza-Olazarán, S., de la O Cavazos, M. E., Villarreal-Pérez, J. Z., Gómez-Govea, M. A., & Garza-González, E. (2018). Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. Epidemiology and Infection, 146(16), 2096-2101. https://doi.org/10.1017/S0950268818002303