Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico

J L Gutiérrez-Ferman, L Villarreal-Treviño, J M Ramírez-Aranda, A Camacho-Ortiz, M R Ballesteros-Elizondo, M R Moreno-Juárez, S Mendoza-Olazarán, M E de la O Cavazos, J Z Villarreal-Pérez, M A Gómez-Govea, E Garza-González

Research output: Contribution to journalArticle

Abstract

We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

Original languageEnglish
Pages (from-to)2096-2101
Number of pages6
JournalEpidemiology and Infection
Volume146
Issue number16
DOIs
Publication statusPublished - Dec 2018
Externally publishedYes

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Bordetella pertussis
Mexico
Whooping Cough
Clone Cells
Pulsed Field Gel Electrophoresis
Genotype
varespladib methyl
Polymerase Chain Reaction
Population
Molecular Epidemiology
Disease Outbreaks
Real-Time Polymerase Chain Reaction
Infection

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Gutiérrez-Ferman, J. L., Villarreal-Treviño, L., Ramírez-Aranda, J. M., Camacho-Ortiz, A., Ballesteros-Elizondo, M. R., Moreno-Juárez, M. R., ... Garza-González, E. (2018). Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. Epidemiology and Infection, 146(16), 2096-2101. https://doi.org/10.1017/S0950268818002303
Gutiérrez-Ferman, J L ; Villarreal-Treviño, L ; Ramírez-Aranda, J M ; Camacho-Ortiz, A ; Ballesteros-Elizondo, M R ; Moreno-Juárez, M R ; Mendoza-Olazarán, S ; de la O Cavazos, M E ; Villarreal-Pérez, J Z ; Gómez-Govea, M A ; Garza-González, E. / Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. In: Epidemiology and Infection. 2018 ; Vol. 146, No. 16. pp. 2096-2101.
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abstract = "We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8{\%}) were positive for pertussis, with 244 (36{\%}) confirmed by both culture and PCR whereas 115 (17{\%}) were positive only by culture and 328 (48{\%}) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55{\%}) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.",
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Gutiérrez-Ferman, JL, Villarreal-Treviño, L, Ramírez-Aranda, JM, Camacho-Ortiz, A, Ballesteros-Elizondo, MR, Moreno-Juárez, MR, Mendoza-Olazarán, S, de la O Cavazos, ME, Villarreal-Pérez, JZ, Gómez-Govea, MA & Garza-González, E 2018, 'Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico', Epidemiology and Infection, vol. 146, no. 16, pp. 2096-2101. https://doi.org/10.1017/S0950268818002303

Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. / Gutiérrez-Ferman, J L; Villarreal-Treviño, L; Ramírez-Aranda, J M; Camacho-Ortiz, A; Ballesteros-Elizondo, M R; Moreno-Juárez, M R; Mendoza-Olazarán, S; de la O Cavazos, M E; Villarreal-Pérez, J Z; Gómez-Govea, M A; Garza-González, E.

In: Epidemiology and Infection, Vol. 146, No. 16, 12.2018, p. 2096-2101.

Research output: Contribution to journalArticle

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T1 - Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico

AU - Gutiérrez-Ferman, J L

AU - Villarreal-Treviño, L

AU - Ramírez-Aranda, J M

AU - Camacho-Ortiz, A

AU - Ballesteros-Elizondo, M R

AU - Moreno-Juárez, M R

AU - Mendoza-Olazarán, S

AU - de la O Cavazos, M E

AU - Villarreal-Pérez, J Z

AU - Gómez-Govea, M A

AU - Garza-González, E

PY - 2018/12

Y1 - 2018/12

N2 - We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

AB - We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

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JO - Epidemiology and Infection

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Gutiérrez-Ferman JL, Villarreal-Treviño L, Ramírez-Aranda JM, Camacho-Ortiz A, Ballesteros-Elizondo MR, Moreno-Juárez MR et al. Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. Epidemiology and Infection. 2018 Dec;146(16):2096-2101. https://doi.org/10.1017/S0950268818002303