TY - JOUR
T1 - Early stage of obesity potentiates nitric oxide reduction during the development of renal failure
AU - Gámez-Méndez, Ana María
AU - Vargas-Robles, Hilda
AU - Arellano-Mendoza, Mónica
AU - Cruz-Laguna, Erika
AU - Rios Rodriguez, Amelia
AU - Escalante Acosta, Bruno Alfonso
PY - 2014/6
Y1 - 2014/6
N2 - BACKGROUND: Obesity is a serious health problem associated with the pathogenesis of various metabolic diseases. Nitric Oxide (NO) plays an important role in kidney function and altered NO levels have been associated with the pathogenesis of obesity. Therefore, we aimed to study whether an early stage of obesity contributes with progression of renal failure through further NO impairment.METHODS: Male C57BL/6 mice were fed with a high-fat diet (HFD) or a normal diet (ND) during 2 weeks. All mice underwent either sham surgery (sham) or 5/6 nephrectomy (Np). One group of HFD Np mice was treated with antioxidants plus L-arginine. Kidney damage parameters were assessed and eNOS metabolism was evaluated.RESULTS: Mice on a HFD increased body weight, eNOS protein and mRNA expression, and radical oxygen species (ROS). Urine nitrites excretion, urine volume, and plasma BH4 were decreased. In HFD mice, 5/6 Np further increased BH2 and urine protein concentration, ROS levels, and eNOS mRNA expression. The decrease in BH4 plasma levels and urine nitrites excretion was accentuated. NO synthesis stimulation with the antioxidants + L-arginine treatment prevented all these changes.CONCLUSIONS: The early changes in NO metabolism are associated with an early stage of obesity. This effect on NO potentiates kidney damage development.
AB - BACKGROUND: Obesity is a serious health problem associated with the pathogenesis of various metabolic diseases. Nitric Oxide (NO) plays an important role in kidney function and altered NO levels have been associated with the pathogenesis of obesity. Therefore, we aimed to study whether an early stage of obesity contributes with progression of renal failure through further NO impairment.METHODS: Male C57BL/6 mice were fed with a high-fat diet (HFD) or a normal diet (ND) during 2 weeks. All mice underwent either sham surgery (sham) or 5/6 nephrectomy (Np). One group of HFD Np mice was treated with antioxidants plus L-arginine. Kidney damage parameters were assessed and eNOS metabolism was evaluated.RESULTS: Mice on a HFD increased body weight, eNOS protein and mRNA expression, and radical oxygen species (ROS). Urine nitrites excretion, urine volume, and plasma BH4 were decreased. In HFD mice, 5/6 Np further increased BH2 and urine protein concentration, ROS levels, and eNOS mRNA expression. The decrease in BH4 plasma levels and urine nitrites excretion was accentuated. NO synthesis stimulation with the antioxidants + L-arginine treatment prevented all these changes.CONCLUSIONS: The early changes in NO metabolism are associated with an early stage of obesity. This effect on NO potentiates kidney damage development.
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U2 - 10.1007/s40620-013-0029-9
DO - 10.1007/s40620-013-0029-9
M3 - Article
C2 - 24446346
SN - 1121-8428
VL - 27
SP - 281
EP - 287
JO - Journal of Nephrology
JF - Journal of Nephrology
IS - 3
ER -
