Dystrophin Dp71 in PC12 cell adhesion

Jose Arturo Enríquez-Aragón, Joel Cerna-Cortés, Mario Bermúdez De León, Francisco García-Sierra, Everardo González, Dominique Mornet, Bulmaro Cisneros

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and β1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to β1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites. © 2005 Lippincott Williams & Wilkins.
Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalNeuroReport
DOIs
Publication statusPublished - 28 Feb 2005
Externally publishedYes

Fingerprint

Dystrophin
PC12 Cells
Cell Adhesion
Integrins
Pseudopodia
Laminin
Nerve Growth Factor
Neurites
Fibronectins
Collagen
Electrons
Staining and Labeling
Proteins

Cite this

Enríquez-Aragón, J. A., Cerna-Cortés, J., Bermúdez De León, M., García-Sierra, F., González, E., Mornet, D., & Cisneros, B. (2005). Dystrophin Dp71 in PC12 cell adhesion. NeuroReport, 235-238. https://doi.org/10.1097/00001756-200502280-00006
Enríquez-Aragón, Jose Arturo ; Cerna-Cortés, Joel ; Bermúdez De León, Mario ; García-Sierra, Francisco ; González, Everardo ; Mornet, Dominique ; Cisneros, Bulmaro. / Dystrophin Dp71 in PC12 cell adhesion. In: NeuroReport. 2005 ; pp. 235-238.
@article{555c04711cd44be799fdd53f88ddd3bb,
title = "Dystrophin Dp71 in PC12 cell adhesion",
abstract = "Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and β1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to β1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites. {\circledC} 2005 Lippincott Williams & Wilkins.",
author = "Enr{\'i}quez-Arag{\'o}n, {Jose Arturo} and Joel Cerna-Cort{\'e}s and {Berm{\'u}dez De Le{\'o}n}, Mario and Francisco Garc{\'i}a-Sierra and Everardo Gonz{\'a}lez and Dominique Mornet and Bulmaro Cisneros",
year = "2005",
month = "2",
day = "28",
doi = "10.1097/00001756-200502280-00006",
language = "English",
pages = "235--238",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",

}

Enríquez-Aragón, JA, Cerna-Cortés, J, Bermúdez De León, M, García-Sierra, F, González, E, Mornet, D & Cisneros, B 2005, 'Dystrophin Dp71 in PC12 cell adhesion', NeuroReport, pp. 235-238. https://doi.org/10.1097/00001756-200502280-00006

Dystrophin Dp71 in PC12 cell adhesion. / Enríquez-Aragón, Jose Arturo; Cerna-Cortés, Joel; Bermúdez De León, Mario; García-Sierra, Francisco; González, Everardo; Mornet, Dominique; Cisneros, Bulmaro.

In: NeuroReport, 28.02.2005, p. 235-238.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dystrophin Dp71 in PC12 cell adhesion

AU - Enríquez-Aragón, Jose Arturo

AU - Cerna-Cortés, Joel

AU - Bermúdez De León, Mario

AU - García-Sierra, Francisco

AU - González, Everardo

AU - Mornet, Dominique

AU - Cisneros, Bulmaro

PY - 2005/2/28

Y1 - 2005/2/28

N2 - Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and β1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to β1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites. © 2005 Lippincott Williams & Wilkins.

AB - Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and β1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to β1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites. © 2005 Lippincott Williams & Wilkins.

U2 - 10.1097/00001756-200502280-00006

DO - 10.1097/00001756-200502280-00006

M3 - Article

SP - 235

EP - 238

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

ER -

Enríquez-Aragón JA, Cerna-Cortés J, Bermúdez De León M, García-Sierra F, González E, Mornet D et al. Dystrophin Dp71 in PC12 cell adhesion. NeuroReport. 2005 Feb 28;235-238. https://doi.org/10.1097/00001756-200502280-00006