Dystrophin Dp71 in PC12 cell adhesion

Jose Arturo Enríquez-Aragón, Joel Cerna-Cortés, Mario Bermúdez De León, Francisco García-Sierra, Everardo González, Dominique Mornet, Bulmaro Cisneros

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Previously, we reported that PC12 cells with decreased Dp71 expression (antisense-Dp71 cells) display deficient nerve-growth-factor-induced neurite outgrowth. In this study, we show that disturbed neurite outgrowth of antisense-Dp71 cells is accompanied by decreased adhesion activity on laminin, collagen and fibronectin. In wild-type cells, the immunostaining of Dp71 and β1-integrin overlaps in the basal area contacting the substrate, but staining of both proteins decrease in the antisense-Dp71 cells. Morphology of antisense-Dp71 cells at the electron microscopic level is characterized by the lack of filopodia, cellular projections involved in adhesion. Our findings suggest that Dp71 is required for the efficient PC12 cell attachment to β1-integrin-dependent substrata and that decreased adhesion activity of the antisense-Dp71 cells could determine their deficiency to extend neurites.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
Issue number3
Publication statusPublished - 28 Feb 2005
Externally publishedYes

Bibliographical note

Copyright 2008 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • General Neuroscience


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