DNA extraction from roots of xonostle

David Montiel, Ernestina Valadez Moctezuma, Guadalupe Palomino, Mario A. Bermúdez, Francisco J. Fernández

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High contents of polysaccharides present in cactus of the genus Opuntia make it difficult to obtain optimal quality DNA for genotyping analysis. In this work, DNA was extracted from the young roots of xoconostle (Opuntia matudae Scheinvar). The quality of the DNA was assessed by random amplification with PCR using RAPD. The extraction method will help to solve technical problems that are commonly reported by different researchers for this group of plants, and it will lead to more efficient handling of samples for DNA analysis. The use of small samples of young roots as an alternative to cladodes, which are normally used for genetic or genomic analysis, was appropriate because high concentrations of DNA with optimum quality were obtained, and this DNA contained low concentrations of the polysaccharides characteristic of this group of cacti. The amplified fragments obtained from the different plants were clear and reproducible.
Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalJournal of the Professional Association for Cactus Development
Publication statusPublished - 1 Dec 2011
Externally publishedYes

Fingerprint

DNA
Opuntia hyptiacantha
polysaccharides
cladodes
Opuntia
genotyping
genetic techniques and protocols
researchers
genomics
sampling
methodology

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Horticulture

Cite this

Montiel, D., Valadez Moctezuma, E., Palomino, G., Bermúdez, M. A., & Fernández, F. J. (2011). DNA extraction from roots of xonostle. Journal of the Professional Association for Cactus Development, 35-40.
Montiel, David ; Valadez Moctezuma, Ernestina ; Palomino, Guadalupe ; Bermúdez, Mario A. ; Fernández, Francisco J. / DNA extraction from roots of xonostle. In: Journal of the Professional Association for Cactus Development. 2011 ; pp. 35-40.
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Montiel, D, Valadez Moctezuma, E, Palomino, G, Bermúdez, MA & Fernández, FJ 2011, 'DNA extraction from roots of xonostle', Journal of the Professional Association for Cactus Development, pp. 35-40.

DNA extraction from roots of xonostle. / Montiel, David; Valadez Moctezuma, Ernestina; Palomino, Guadalupe; Bermúdez, Mario A.; Fernández, Francisco J.

In: Journal of the Professional Association for Cactus Development, 01.12.2011, p. 35-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA extraction from roots of xonostle

AU - Montiel, David

AU - Valadez Moctezuma, Ernestina

AU - Palomino, Guadalupe

AU - Bermúdez, Mario A.

AU - Fernández, Francisco J.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - High contents of polysaccharides present in cactus of the genus Opuntia make it difficult to obtain optimal quality DNA for genotyping analysis. In this work, DNA was extracted from the young roots of xoconostle (Opuntia matudae Scheinvar). The quality of the DNA was assessed by random amplification with PCR using RAPD. The extraction method will help to solve technical problems that are commonly reported by different researchers for this group of plants, and it will lead to more efficient handling of samples for DNA analysis. The use of small samples of young roots as an alternative to cladodes, which are normally used for genetic or genomic analysis, was appropriate because high concentrations of DNA with optimum quality were obtained, and this DNA contained low concentrations of the polysaccharides characteristic of this group of cacti. The amplified fragments obtained from the different plants were clear and reproducible.

AB - High contents of polysaccharides present in cactus of the genus Opuntia make it difficult to obtain optimal quality DNA for genotyping analysis. In this work, DNA was extracted from the young roots of xoconostle (Opuntia matudae Scheinvar). The quality of the DNA was assessed by random amplification with PCR using RAPD. The extraction method will help to solve technical problems that are commonly reported by different researchers for this group of plants, and it will lead to more efficient handling of samples for DNA analysis. The use of small samples of young roots as an alternative to cladodes, which are normally used for genetic or genomic analysis, was appropriate because high concentrations of DNA with optimum quality were obtained, and this DNA contained low concentrations of the polysaccharides characteristic of this group of cacti. The amplified fragments obtained from the different plants were clear and reproducible.

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JO - Journal of the Professional Association for Cactus Development

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Montiel D, Valadez Moctezuma E, Palomino G, Bermúdez MA, Fernández FJ. DNA extraction from roots of xonostle. Journal of the Professional Association for Cactus Development. 2011 Dec 1;35-40.