Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues

Qingmeng Pi, Sushila Maharjan, Xiang Yan, Xiao Liu, Bijay Singh, Anne Metje van Genderen, Felipe Robledo-Padilla, Roberto Parra-Saldivar, Ning Hu, Weitao Jia, Changliang Xu, Jian Kang, Shabir Hassan, Haibo Cheng, Xu Hou, Ali Khademhosseini, Yu Shrike Zhang

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Despite advances in the bioprinting technology, biofabrication of circumferentially multilayered tubular tissues or organs with cellular heterogeneity, such as blood vessels, trachea, intestine, colon, ureter, and urethra, remains a challenge. Herein, a promising multichannel coaxial extrusion system (MCCES) for microfluidic bioprinting of circumferentially multilayered tubular tissues in a single step, using customized bioinks constituting gelatin methacryloyl, alginate, and eight-arm poly(ethylene glycol) acrylate with a tripentaerythritol core, is presented. These perfusable cannular constructs can be continuously tuned up from monolayer to triple layers at regular intervals across the length of a bioprinted tube. Using customized bioink and MCCES, bioprinting of several tubular tissue constructs using relevant cell types with adequate biofunctionality including cell viability, proliferation, and differentiation is demonstrated. Specifically, cannular urothelial tissue constructs are bioprinted, using human urothelial cells and human bladder smooth muscle cells, as well as vascular tissue constructs, using human umbilical vein endothelial cells and human smooth muscle cells. These bioprinted cannular tissues can be actively perfused with fluids and nutrients to promote growth and proliferation of the embedded cell types. The fabrication of such tunable and perfusable circumferentially multilayered tissues represents a fundamental step toward creating human cannular tissues.

Original languageEnglish
Article number1706913
Pages (from-to)e1706913
JournalAdvanced Materials
Volume30
Issue number43
DOIs
Publication statusPublished - 25 Oct 2018
Externally publishedYes

Bibliographical note

Funding Information:
Q.P., S.M., X.Y., and X.L. contributed equally to this work. The authors acknowledge funding from the National Institutes of Health (AR057837, DE021468, AR068258, AR066193, EB022403, EB021148, HL137193, EB021857, and EB024403) and the Presidential Early Career Award for Scientists and Engineers (PECASE). Q.P. gratefully acknowledges funding by China Scholarship Council (No. 201406235036). S.M. acknowledges New England Anti-Vivisection Society (NEAVS) and the American Fund for Alternatives to Animal Research (AFAAR) for Postdoctoral Fellowship. X.L. acknowledges funding by the National Natural Science Foundation of China (No.31570947). X.Y. acknowledges funding by the National Natural Science Foundation of China(No. 81772712). X.H. acknowledges funding by the National Key Research and Development Program of China (Project No. 2018YFA0209500), the National Natural Science Foundation of China (21673197, 21621091), Young Overseas High-level Talents Introduction Plan, the 111 Project (B16029), the Natural Science Foundation of Fujian Province of China (No. 2018J06003) and Special Project of Strategic Emerging Industries from Fujian Development and Reform Commission. R.P.S acknowledges funding by Tecnologico de Monterrey and MIT Nanotechnology Program. F.R.P. was supported by the CONACYT postdoctoral fellowship (Grant No. 291018-173853). S.H. acknowledges SNSF for Early Mobility postdoctoral fellowship. Y.S.Z. acknowledges funding from the National Cancer Institute of the National Institutes of Health (K99CA201603) and the NEAVS. The authors would like to thank Prof. Samir Mitragotri for his generous help on microscopy.

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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