Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues

Qingmeng Pi, Sushila Maharjan, Xiang Yan, Xiao Liu, Bijay Singh, Anne Metje van Genderen, Felipe Robledo-Padilla, Roberto Parra-Saldivar, Ning Hu, Weitao Jia, Changliang Xu, Jian Kang, Shabir Hassan, Haibo Cheng, Xu Hou, Ali Khademhosseini, Yu Shrike Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Despite advances in the bioprinting technology, biofabrication of circumferentially multilayered tubular tissues or organs with cellular heterogeneity, such as blood vessels, trachea, intestine, colon, ureter, and urethra, remains a challenge. Herein, a promising multichannel coaxial extrusion system (MCCES) for microfluidic bioprinting of circumferentially multilayered tubular tissues in a single step, using customized bioinks constituting gelatin methacryloyl, alginate, and eight-arm poly(ethylene glycol) acrylate with a tripentaerythritol core, is presented. These perfusable cannular constructs can be continuously tuned up from monolayer to triple layers at regular intervals across the length of a bioprinted tube. Using customized bioink and MCCES, bioprinting of several tubular tissue constructs using relevant cell types with adequate biofunctionality including cell viability, proliferation, and differentiation is demonstrated. Specifically, cannular urothelial tissue constructs are bioprinted, using human urothelial cells and human bladder smooth muscle cells, as well as vascular tissue constructs, using human umbilical vein endothelial cells and human smooth muscle cells. These bioprinted cannular tissues can be actively perfused with fluids and nutrients to promote growth and proliferation of the embedded cell types. The fabrication of such tunable and perfusable circumferentially multilayered tissues represents a fundamental step toward creating human cannular tissues.

Original languageEnglish
Article number1706913
JournalAdvanced Materials
Volume30
Issue number43
DOIs
Publication statusPublished - 25 Oct 2018
Externally publishedYes

Fingerprint

Microfluidics
Tissue
Extrusion
Muscle
Cells
Alginate
Endothelial cells
Blood vessels
Cell proliferation
Gelatin
Polyethylene glycols
Nutrients
Monolayers
Fabrication
Fluids

Bibliographical note

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Pi, Q., Maharjan, S., Yan, X., Liu, X., Singh, B., van Genderen, A. M., ... Zhang, Y. S. (2018). Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues. Advanced Materials, 30(43), [1706913]. https://doi.org/10.1002/adma.201706913
Pi, Qingmeng ; Maharjan, Sushila ; Yan, Xiang ; Liu, Xiao ; Singh, Bijay ; van Genderen, Anne Metje ; Robledo-Padilla, Felipe ; Parra-Saldivar, Roberto ; Hu, Ning ; Jia, Weitao ; Xu, Changliang ; Kang, Jian ; Hassan, Shabir ; Cheng, Haibo ; Hou, Xu ; Khademhosseini, Ali ; Zhang, Yu Shrike. / Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues. In: Advanced Materials. 2018 ; Vol. 30, No. 43.
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abstract = "Despite advances in the bioprinting technology, biofabrication of circumferentially multilayered tubular tissues or organs with cellular heterogeneity, such as blood vessels, trachea, intestine, colon, ureter, and urethra, remains a challenge. Herein, a promising multichannel coaxial extrusion system (MCCES) for microfluidic bioprinting of circumferentially multilayered tubular tissues in a single step, using customized bioinks constituting gelatin methacryloyl, alginate, and eight-arm poly(ethylene glycol) acrylate with a tripentaerythritol core, is presented. These perfusable cannular constructs can be continuously tuned up from monolayer to triple layers at regular intervals across the length of a bioprinted tube. Using customized bioink and MCCES, bioprinting of several tubular tissue constructs using relevant cell types with adequate biofunctionality including cell viability, proliferation, and differentiation is demonstrated. Specifically, cannular urothelial tissue constructs are bioprinted, using human urothelial cells and human bladder smooth muscle cells, as well as vascular tissue constructs, using human umbilical vein endothelial cells and human smooth muscle cells. These bioprinted cannular tissues can be actively perfused with fluids and nutrients to promote growth and proliferation of the embedded cell types. The fabrication of such tunable and perfusable circumferentially multilayered tissues represents a fundamental step toward creating human cannular tissues.",
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Pi, Q, Maharjan, S, Yan, X, Liu, X, Singh, B, van Genderen, AM, Robledo-Padilla, F, Parra-Saldivar, R, Hu, N, Jia, W, Xu, C, Kang, J, Hassan, S, Cheng, H, Hou, X, Khademhosseini, A & Zhang, YS 2018, 'Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues', Advanced Materials, vol. 30, no. 43, 1706913. https://doi.org/10.1002/adma.201706913

Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues. / Pi, Qingmeng; Maharjan, Sushila; Yan, Xiang; Liu, Xiao; Singh, Bijay; van Genderen, Anne Metje; Robledo-Padilla, Felipe; Parra-Saldivar, Roberto; Hu, Ning; Jia, Weitao; Xu, Changliang; Kang, Jian; Hassan, Shabir; Cheng, Haibo; Hou, Xu; Khademhosseini, Ali; Zhang, Yu Shrike.

In: Advanced Materials, Vol. 30, No. 43, 1706913, 25.10.2018.

Research output: Contribution to journalArticle

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AU - Pi, Qingmeng

AU - Maharjan, Sushila

AU - Yan, Xiang

AU - Liu, Xiao

AU - Singh, Bijay

AU - van Genderen, Anne Metje

AU - Robledo-Padilla, Felipe

AU - Parra-Saldivar, Roberto

AU - Hu, Ning

AU - Jia, Weitao

AU - Xu, Changliang

AU - Kang, Jian

AU - Hassan, Shabir

AU - Cheng, Haibo

AU - Hou, Xu

AU - Khademhosseini, Ali

AU - Zhang, Yu Shrike

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Y1 - 2018/10/25

N2 - Despite advances in the bioprinting technology, biofabrication of circumferentially multilayered tubular tissues or organs with cellular heterogeneity, such as blood vessels, trachea, intestine, colon, ureter, and urethra, remains a challenge. Herein, a promising multichannel coaxial extrusion system (MCCES) for microfluidic bioprinting of circumferentially multilayered tubular tissues in a single step, using customized bioinks constituting gelatin methacryloyl, alginate, and eight-arm poly(ethylene glycol) acrylate with a tripentaerythritol core, is presented. These perfusable cannular constructs can be continuously tuned up from monolayer to triple layers at regular intervals across the length of a bioprinted tube. Using customized bioink and MCCES, bioprinting of several tubular tissue constructs using relevant cell types with adequate biofunctionality including cell viability, proliferation, and differentiation is demonstrated. Specifically, cannular urothelial tissue constructs are bioprinted, using human urothelial cells and human bladder smooth muscle cells, as well as vascular tissue constructs, using human umbilical vein endothelial cells and human smooth muscle cells. These bioprinted cannular tissues can be actively perfused with fluids and nutrients to promote growth and proliferation of the embedded cell types. The fabrication of such tunable and perfusable circumferentially multilayered tissues represents a fundamental step toward creating human cannular tissues.

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Pi Q, Maharjan S, Yan X, Liu X, Singh B, van Genderen AM et al. Digitally Tunable Microfluidic Bioprinting of Multilayered Cannular Tissues. Advanced Materials. 2018 Oct 25;30(43). 1706913. https://doi.org/10.1002/adma.201706913