Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates

Laura González-Escalante, Katia Peñuelas-Urquides, Salvador Said-Fernández, Beatriz Silva-Ramírez, Mario Bermúdez de León

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

© FEMS 2015. All rights reserved. Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant clinical isolate. Here, we have evaluated the expression of these genes in 24 clinical isolates that belong to different lineages and have different drug resistance profiles. In vitro, growth kinetics analysis showed no difference in the growth of the clinical isolates, and thus drug resistance occurred without a fitness cost. However, a quantitative reverse transcription PCR analysis of gene expression revealed high variability among the clinical isolates, including those with similar drug resistance profiles. Due to the complexity of gene regulation pathways and the wide diversity of M. tuberculosis lineages, the use of gene expression as a molecular signature for drug resistance is not straightforward. Therefore, we recommend that the expression of M. tuberculosis genes be performed individually, and baseline expression levels should be verified among several different clinical isolates, before any further applications of these findings.
Original languageEnglish
JournalFEMS Microbiology Letters
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

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Mycobacterium tuberculosis
Drug Resistance
Gene Expression
Genes
Growth
Reverse Transcription
Costs and Cost Analysis
Polymerase Chain Reaction
Mutation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

González-Escalante, Laura ; Peñuelas-Urquides, Katia ; Said-Fernández, Salvador ; Silva-Ramírez, Beatriz ; de León, Mario Bermúdez. / Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates. In: FEMS Microbiology Letters. 2015.
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Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates. / González-Escalante, Laura; Peñuelas-Urquides, Katia; Said-Fernández, Salvador; Silva-Ramírez, Beatriz; de León, Mario Bermúdez.

In: FEMS Microbiology Letters, 01.12.2015.

Research output: Contribution to journalArticle

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AB - © FEMS 2015. All rights reserved. Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant clinical isolate. Here, we have evaluated the expression of these genes in 24 clinical isolates that belong to different lineages and have different drug resistance profiles. In vitro, growth kinetics analysis showed no difference in the growth of the clinical isolates, and thus drug resistance occurred without a fitness cost. However, a quantitative reverse transcription PCR analysis of gene expression revealed high variability among the clinical isolates, including those with similar drug resistance profiles. Due to the complexity of gene regulation pathways and the wide diversity of M. tuberculosis lineages, the use of gene expression as a molecular signature for drug resistance is not straightforward. Therefore, we recommend that the expression of M. tuberculosis genes be performed individually, and baseline expression levels should be verified among several different clinical isolates, before any further applications of these findings.

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