Detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis patients by multiplex PCR.

Myriam A. De La Garza-Ramos, Luis J. Galán-Wong, Raúl G. Caffesse, Francisco González-Salazar, Benito Pereyra-Alférez

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A Multiplex PCR assay for the detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis is presented. A total of 180 samples from 65 adults with untreated periodontitis and 17 healthy volunteers were taken and processed in a simple boiling step. Cell lysates were used as DNA source for multiplex PCR assays. Primers were designed from 16S rRNA gene sequences from the GenBank-EMBL database showing specificity for target pathogens. This multiplex PCR system could detect 8.2 P gingivalis and S. intermedius cells. In untreated periodontitis patients, only 78.5% were positive for one or both bacteria; 37% were positive for P gingivalis only, 17% for S. intermedius and 24.5% for both. P. gingivalis was detected in 23.5% of healthy volunteers, while S. intermedius was not detected in the same patients. The distribution of these bacteria was related to the periodontal probing depth, while 95.23% of patients with pockets wih 6 to 7 mm deep were positive for either or both, only 70.45% of of them with 4 to 5 mm pockets were positive.
Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalActa odontológica latinoamericana : AOL
Publication statusPublished - 1 Dec 2008
Externally publishedYes

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Streptococcus intermedius
Chronic Periodontitis
Porphyromonas gingivalis
Multiplex Polymerase Chain Reaction
Periodontitis
Healthy Volunteers
Bacteria
Nucleic Acid Databases
rRNA Genes
Databases
DNA

Cite this

De La Garza-Ramos, Myriam A. ; Galán-Wong, Luis J. ; Caffesse, Raúl G. ; González-Salazar, Francisco ; Pereyra-Alférez, Benito. / Detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis patients by multiplex PCR. In: Acta odontológica latinoamericana : AOL. 2008 ; pp. 163-167.
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abstract = "A Multiplex PCR assay for the detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis is presented. A total of 180 samples from 65 adults with untreated periodontitis and 17 healthy volunteers were taken and processed in a simple boiling step. Cell lysates were used as DNA source for multiplex PCR assays. Primers were designed from 16S rRNA gene sequences from the GenBank-EMBL database showing specificity for target pathogens. This multiplex PCR system could detect 8.2 P gingivalis and S. intermedius cells. In untreated periodontitis patients, only 78.5{\%} were positive for one or both bacteria; 37{\%} were positive for P gingivalis only, 17{\%} for S. intermedius and 24.5{\%} for both. P. gingivalis was detected in 23.5{\%} of healthy volunteers, while S. intermedius was not detected in the same patients. The distribution of these bacteria was related to the periodontal probing depth, while 95.23{\%} of patients with pockets wih 6 to 7 mm deep were positive for either or both, only 70.45{\%} of of them with 4 to 5 mm pockets were positive.",
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Detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis patients by multiplex PCR. / De La Garza-Ramos, Myriam A.; Galán-Wong, Luis J.; Caffesse, Raúl G.; González-Salazar, Francisco; Pereyra-Alférez, Benito.

In: Acta odontológica latinoamericana : AOL, 01.12.2008, p. 163-167.

Research output: Contribution to journalArticle

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T1 - Detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis patients by multiplex PCR.

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AU - González-Salazar, Francisco

AU - Pereyra-Alférez, Benito

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AB - A Multiplex PCR assay for the detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis is presented. A total of 180 samples from 65 adults with untreated periodontitis and 17 healthy volunteers were taken and processed in a simple boiling step. Cell lysates were used as DNA source for multiplex PCR assays. Primers were designed from 16S rRNA gene sequences from the GenBank-EMBL database showing specificity for target pathogens. This multiplex PCR system could detect 8.2 P gingivalis and S. intermedius cells. In untreated periodontitis patients, only 78.5% were positive for one or both bacteria; 37% were positive for P gingivalis only, 17% for S. intermedius and 24.5% for both. P. gingivalis was detected in 23.5% of healthy volunteers, while S. intermedius was not detected in the same patients. The distribution of these bacteria was related to the periodontal probing depth, while 95.23% of patients with pockets wih 6 to 7 mm deep were positive for either or both, only 70.45% of of them with 4 to 5 mm pockets were positive.

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