TY - JOUR
T1 - Detection of Helicobacter pylori in dental plaque of Mexican children by real-time PCR
AU - González Salazar, Francisco
AU - Valdez-Gonzalez, Jose Amadeo
AU - De La Garza-Ramos, Myriam A.
AU - Vargas-Villarreal, Javier
PY - 2014
Y1 - 2014
N2 - Dental plaque in adult patients is well identified as a\r\nreservoir for Helicobacter pylori. This\r\nquestion still remains unclear in children. The aim of this study is to\r\nidentify the presence of this bacterium in dental plaque of Mexican pediatric\r\npatients, using Real Time Polymerase Chain\r\nReaction (qPCR). Forty patients from 2 to 11 years without dyspeptic\r\nsymptoms were enrolled. Samples were collected from the subgingival space of\r\nthe lingual side of the lower molars and cultured in selective medium. Therefore, qPCR analysis was conducted. According to\r\nthe results obtained in this study, it was found that 35% of the pediatric population who participated tested qPCR positive for the presence of H. pylori in dental plaque samples. No significant\r\nassociations were detected among isolation\r\nrate by gender or age. We found that dental plaque may be a reservoir for H. pylori. However, more research is needed to establish the way of the\r\ninfection of pediatric population.
AB - Dental plaque in adult patients is well identified as a\r\nreservoir for Helicobacter pylori. This\r\nquestion still remains unclear in children. The aim of this study is to\r\nidentify the presence of this bacterium in dental plaque of Mexican pediatric\r\npatients, using Real Time Polymerase Chain\r\nReaction (qPCR). Forty patients from 2 to 11 years without dyspeptic\r\nsymptoms were enrolled. Samples were collected from the subgingival space of\r\nthe lingual side of the lower molars and cultured in selective medium. Therefore, qPCR analysis was conducted. According to\r\nthe results obtained in this study, it was found that 35% of the pediatric population who participated tested qPCR positive for the presence of H. pylori in dental plaque samples. No significant\r\nassociations were detected among isolation\r\nrate by gender or age. We found that dental plaque may be a reservoir for H. pylori. However, more research is needed to establish the way of the\r\ninfection of pediatric population.
UR - http://www.mendeley.com/research/detection-ltigthelicobacter-pyloriltigt-dental-plaque-mexican-children-realtime-pcr
U2 - 10.4236/health.2014.64034
DO - 10.4236/health.2014.64034
M3 - Article
SN - 1949-4998
VL - 06
SP - 231
EP - 235
JO - Health
JF - Health
IS - 04
ER -