CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico

Fabiola Castorena-Torres, Ania Mendoza-Cantú, Mario Bermúdez De León, Bulmaro Cisneros, Omar Zapata-Pérez, Lizbeth López-Carrillo, Juan E. Salinas, Arnulfo Albores

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity. © 2004 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalToxicology Letters
DOIs
Publication statusPublished - 28 Apr 2005
Externally publishedYes

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Cytochrome P-450 CYP1A2
5' Flanking Region
Polycyclic Aromatic Hydrocarbons
Mexico
Polymorphism
Introns
Genotype
Caffeine
Phenotype
Population
Ireland
Volunteers
Enzyme activity
Enzymes

Cite this

Castorena-Torres, F., Mendoza-Cantú, A., De León, M. B., Cisneros, B., Zapata-Pérez, O., López-Carrillo, L., ... Albores, A. (2005). CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico. Toxicology Letters, 331-339. https://doi.org/10.1016/j.toxlet.2004.12.005
Castorena-Torres, Fabiola ; Mendoza-Cantú, Ania ; De León, Mario Bermúdez ; Cisneros, Bulmaro ; Zapata-Pérez, Omar ; López-Carrillo, Lizbeth ; Salinas, Juan E. ; Albores, Arnulfo. / CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico. In: Toxicology Letters. 2005 ; pp. 331-339.
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author = "Fabiola Castorena-Torres and Ania Mendoza-Cant{\'u} and {De Le{\'o}n}, {Mario Berm{\'u}dez} and Bulmaro Cisneros and Omar Zapata-P{\'e}rez and Lizbeth L{\'o}pez-Carrillo and Salinas, {Juan E.} and Arnulfo Albores",
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Castorena-Torres, F, Mendoza-Cantú, A, De León, MB, Cisneros, B, Zapata-Pérez, O, López-Carrillo, L, Salinas, JE & Albores, A 2005, 'CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico', Toxicology Letters, pp. 331-339. https://doi.org/10.1016/j.toxlet.2004.12.005

CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico. / Castorena-Torres, Fabiola; Mendoza-Cantú, Ania; De León, Mario Bermúdez; Cisneros, Bulmaro; Zapata-Pérez, Omar; López-Carrillo, Lizbeth; Salinas, Juan E.; Albores, Arnulfo.

In: Toxicology Letters, 28.04.2005, p. 331-339.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico

AU - Castorena-Torres, Fabiola

AU - Mendoza-Cantú, Ania

AU - De León, Mario Bermúdez

AU - Cisneros, Bulmaro

AU - Zapata-Pérez, Omar

AU - López-Carrillo, Lizbeth

AU - Salinas, Juan E.

AU - Albores, Arnulfo

PY - 2005/4/28

Y1 - 2005/4/28

N2 - CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity. © 2004 Elsevier Ireland Ltd. All rights reserved.

AB - CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity. © 2004 Elsevier Ireland Ltd. All rights reserved.

U2 - 10.1016/j.toxlet.2004.12.005

DO - 10.1016/j.toxlet.2004.12.005

M3 - Article

SP - 331

EP - 339

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

ER -

Castorena-Torres F, Mendoza-Cantú A, De León MB, Cisneros B, Zapata-Pérez O, López-Carrillo L et al. CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico. Toxicology Letters. 2005 Apr 28;331-339. https://doi.org/10.1016/j.toxlet.2004.12.005