Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 331-339 |
| Number of pages | 9 |
| Journal | Toxicology Letters |
| DOIs | |
| Publication status | Published - 28 Apr 2005 |
| Externally published | Yes |
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CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico. / Castorena-Torres, Fabiola; Mendoza-Cantú, Ania; De León, Mario Bermúdez; Cisneros, Bulmaro; Zapata-Pérez, Omar; López-Carrillo, Lizbeth; Salinas, Juan E.; Albores, Arnulfo.
In: Toxicology Letters, 28.04.2005, p. 331-339.Research output: Contribution to journal › Article
TY - JOUR
T1 - CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico
AU - Castorena-Torres, Fabiola
AU - Mendoza-Cantú, Ania
AU - De León, Mario Bermúdez
AU - Cisneros, Bulmaro
AU - Zapata-Pérez, Omar
AU - López-Carrillo, Lizbeth
AU - Salinas, Juan E.
AU - Albores, Arnulfo
PY - 2005/4/28
Y1 - 2005/4/28
N2 - CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity. © 2004 Elsevier Ireland Ltd. All rights reserved.
AB - CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity. © 2004 Elsevier Ireland Ltd. All rights reserved.
U2 - 10.1016/j.toxlet.2004.12.005
DO - 10.1016/j.toxlet.2004.12.005
M3 - Article
SP - 331
EP - 339
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
ER -