CYP1A2 phenotype and genotype in a population from the Carboniferous Region of Coahuila, Mexico

Fabiola Castorena-Torres, Ania Mendoza-Cantú, Mario Bermúdez De León, Bulmaro Cisneros, Omar Zapata-Pérez, Lizbeth López-Carrillo, Juan E. Salinas, Arnulfo Albores

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36 Citations (Scopus)

Abstract

CYP1A2 regulation by polycyclic aromatic hydrocarbons (PAHs) exposure and polymorphism was investigated in 46 male volunteers from the Carboniferous Region in northern Coahuila, Mexico. PAH exposure was estimated by the urinary excretion of 1-hydroxypyrene (1-OHP), whereas the regulatory effects were assessed by the caffeine metabolic ratio (CMR). Genotype was evaluated by determining 5′-flanking region (-2964) and intron I (734) polymorphisms. A statistically significant difference in the urinary 1-OHP geometric means of Barroterán, Cloete and Juárez (2.30, 0.45 and 0.04, respectively) was observed. As for the genotype, the intron I distribution was 0% C/C, 46% C/A and 54% A/A, whereas that of the 5′-flanking region was 26% G/G, 42% G/A and 32% A/A. Both distributions were in agreement with the Hardy-Weinberg equilibrium model. A greater enzyme activity was observed in the A/A compared to C/A individuals according to the CMR (P < 0.001), whereas the 5′-flanking region polymorphism showed no effect on CYP1A2 enzymatic activity. These results suggest that intron I polymorphism and PAH exposure are relevant factors that modulate CYP1A2 enzymatic activity.

Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalToxicology Letters
Volume156
Issue number3
DOIs
Publication statusPublished - 28 Apr 2005
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by: EC contract: ERB IC18-CT980341. FCT was supported by a CONACyT grant. We gratefully acknowledge R. Reyes and V. Ruíz-Pérez for technical assistance in 1-OHP quantitation, N. Cirnes for artistic work, and Dr. J.R. Bend and L. Sarkissian for their critical review of this manuscript.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

  • Toxicology

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