Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A pectin (CAP) was extracted from the husk of Cicer arietinum L..
Monosaccharide analysis of CAP revealed the dominance of galacturonic acid and smaller
amounts of galactose, arabinose, rhamnose, glucose, xylose and mannose. Viscosimetric
analysis showed that the intrinsic viscosity ([η]) and the molecular weight (MW) of CAP
were 296 mL/g and 105 kDa, respectively. The degree of esterification (DE = 10%) was
determined by FTIR spectroscopy. CAP exhibited a dose-dependent free radical
scavenging activity, as shown by its DPPH radical inhibition. At 1.0 mg/mL CAP exhibited
a scavenging rate of 29% on DPPH radicals. The evaluation of antioxidant activity
suggested that CAP had good potential for DPPH radical scavenging activity and should be
explored as a novel potential antioxidant.
Original languageEnglish
Pages (from-to)6948-6955
Number of pages8
JournalMolecules
Volume15
Publication statusPublished - 15 Jun 2010

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Cicer
Scavenging
scavenging
free radicals
Free Radicals
Antioxidants
antioxidants
Rhamnose
Arabinose
Monosaccharides
Esterification
Fourier Transform Infrared Spectroscopy
Mannose
Galactose
Viscosity
xylose
monosaccharides
galactose
Spectrum Analysis
Molecular Weight

Cite this

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title = "Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin",
abstract = "A pectin (CAP) was extracted from the husk of Cicer arietinum L..Monosaccharide analysis of CAP revealed the dominance of galacturonic acid and smalleramounts of galactose, arabinose, rhamnose, glucose, xylose and mannose. Viscosimetricanalysis showed that the intrinsic viscosity ([η]) and the molecular weight (MW) of CAPwere 296 mL/g and 105 kDa, respectively. The degree of esterification (DE = 10{\%}) wasdetermined by FTIR spectroscopy. CAP exhibited a dose-dependent free radicalscavenging activity, as shown by its DPPH radical inhibition. At 1.0 mg/mL CAP exhibiteda scavenging rate of 29{\%} on DPPH radicals. The evaluation of antioxidant activitysuggested that CAP had good potential for DPPH radical scavenging activity and should beexplored as a novel potential antioxidant.",
author = "{Huerta Oros}, Joselina",
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journal = "Molecules",
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Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin. / Huerta Oros, Joselina.

In: Molecules, Vol. 15, 15.06.2010, p. 6948-6955.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin

AU - Huerta Oros, Joselina

PY - 2010/6/15

Y1 - 2010/6/15

N2 - A pectin (CAP) was extracted from the husk of Cicer arietinum L..Monosaccharide analysis of CAP revealed the dominance of galacturonic acid and smalleramounts of galactose, arabinose, rhamnose, glucose, xylose and mannose. Viscosimetricanalysis showed that the intrinsic viscosity ([η]) and the molecular weight (MW) of CAPwere 296 mL/g and 105 kDa, respectively. The degree of esterification (DE = 10%) wasdetermined by FTIR spectroscopy. CAP exhibited a dose-dependent free radicalscavenging activity, as shown by its DPPH radical inhibition. At 1.0 mg/mL CAP exhibiteda scavenging rate of 29% on DPPH radicals. The evaluation of antioxidant activitysuggested that CAP had good potential for DPPH radical scavenging activity and should beexplored as a novel potential antioxidant.

AB - A pectin (CAP) was extracted from the husk of Cicer arietinum L..Monosaccharide analysis of CAP revealed the dominance of galacturonic acid and smalleramounts of galactose, arabinose, rhamnose, glucose, xylose and mannose. Viscosimetricanalysis showed that the intrinsic viscosity ([η]) and the molecular weight (MW) of CAPwere 296 mL/g and 105 kDa, respectively. The degree of esterification (DE = 10%) wasdetermined by FTIR spectroscopy. CAP exhibited a dose-dependent free radicalscavenging activity, as shown by its DPPH radical inhibition. At 1.0 mg/mL CAP exhibiteda scavenging rate of 29% on DPPH radicals. The evaluation of antioxidant activitysuggested that CAP had good potential for DPPH radical scavenging activity and should beexplored as a novel potential antioxidant.

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JF - Molecules

SN - 1420-3049

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