Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma

Fernando Martínez-Pérez, Claudia María García-Cuellar, Rene Hernandez-Delgadillo, Valentin Zaragoza-Magaña, Yesennia Sánchez-Pérez, Irene Meester, Sergio Eduardo Nakagoshi-Cepeda, Juan Manuel Solís-Soto, María Argelia Akemi Nakagoshi-Cepeda, Shankararaman Chellam, Claudio Cabral-Romero

Research output: Contribution to journalArticle

Abstract

The objective of this study was to compare the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine (CHX) on human squamous cell carcinoma. BisBAL NPs were synthesized by colloidal method and characterized by energy dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (EDS-SEM). The effect of BisBAL NPs and CHX on oral cancer cell line (CAL-27) and nontumor control cell human gingival fibroblasts (HGFs) was determined by MTT cell viability assay. The obtained results showed selective inhibition of CAL-27 cell growth by BisBAL nanoclusters. A 24 h exposition to 25 μM BisBAL NP decreased 91% of CAL-27 cell growth, while nontumor HGFs cells were unaffected by BisBAL NPs showing 90% of cell viability. In contrast, CHX kills both CAL-27 and HGFs with the same efficacy. 25 μM of CHX decreased 97% and 80% of tumor and nontumoral cell growth. BisBAL NP and CHX alter cell permeability suggesting that action mechanism may include loss of cell membrane integrity. Also, CHX and not BisBAL NP presented genotoxicity on genomic DNA of tumor cells. As conclusion, BisBAL NPs have a selective antitumor activity on human squamous cell carcinoma, unlike CHX which was cytotoxic for both tumoral and nontumoral control cells.

Original languageEnglish
Article number8148219
JournalJournal of Nanomaterials
Volume2019
DOIs
Publication statusPublished - 1 Jan 2019

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Bismuth
Chlorhexidine
Cell growth
Fibroblasts
Cells
Nanoparticles
Tumors
Scanning electron microscopy
Nanoclusters
Cell membranes
Energy dispersive spectroscopy
Assays
DNA
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Martínez-Pérez, F., García-Cuellar, C. M., Hernandez-Delgadillo, R., Zaragoza-Magaña, V., Sánchez-Pérez, Y., Meester, I., ... Cabral-Romero, C. (2019). Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma. Journal of Nanomaterials, 2019, [8148219]. https://doi.org/10.1155/2019/8148219
Martínez-Pérez, Fernando ; García-Cuellar, Claudia María ; Hernandez-Delgadillo, Rene ; Zaragoza-Magaña, Valentin ; Sánchez-Pérez, Yesennia ; Meester, Irene ; Nakagoshi-Cepeda, Sergio Eduardo ; Solís-Soto, Juan Manuel ; Nakagoshi-Cepeda, María Argelia Akemi ; Chellam, Shankararaman ; Cabral-Romero, Claudio. / Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma. In: Journal of Nanomaterials. 2019 ; Vol. 2019.
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abstract = "The objective of this study was to compare the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine (CHX) on human squamous cell carcinoma. BisBAL NPs were synthesized by colloidal method and characterized by energy dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (EDS-SEM). The effect of BisBAL NPs and CHX on oral cancer cell line (CAL-27) and nontumor control cell human gingival fibroblasts (HGFs) was determined by MTT cell viability assay. The obtained results showed selective inhibition of CAL-27 cell growth by BisBAL nanoclusters. A 24 h exposition to 25 μM BisBAL NP decreased 91{\%} of CAL-27 cell growth, while nontumor HGFs cells were unaffected by BisBAL NPs showing 90{\%} of cell viability. In contrast, CHX kills both CAL-27 and HGFs with the same efficacy. 25 μM of CHX decreased 97{\%} and 80{\%} of tumor and nontumoral cell growth. BisBAL NP and CHX alter cell permeability suggesting that action mechanism may include loss of cell membrane integrity. Also, CHX and not BisBAL NP presented genotoxicity on genomic DNA of tumor cells. As conclusion, BisBAL NPs have a selective antitumor activity on human squamous cell carcinoma, unlike CHX which was cytotoxic for both tumoral and nontumoral control cells.",
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Martínez-Pérez, F, García-Cuellar, CM, Hernandez-Delgadillo, R, Zaragoza-Magaña, V, Sánchez-Pérez, Y, Meester, I, Nakagoshi-Cepeda, SE, Solís-Soto, JM, Nakagoshi-Cepeda, MAA, Chellam, S & Cabral-Romero, C 2019, 'Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma', Journal of Nanomaterials, vol. 2019, 8148219. https://doi.org/10.1155/2019/8148219

Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma. / Martínez-Pérez, Fernando; García-Cuellar, Claudia María; Hernandez-Delgadillo, Rene; Zaragoza-Magaña, Valentin; Sánchez-Pérez, Yesennia; Meester, Irene; Nakagoshi-Cepeda, Sergio Eduardo; Solís-Soto, Juan Manuel; Nakagoshi-Cepeda, María Argelia Akemi; Chellam, Shankararaman; Cabral-Romero, Claudio.

In: Journal of Nanomaterials, Vol. 2019, 8148219, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma

AU - Martínez-Pérez, Fernando

AU - García-Cuellar, Claudia María

AU - Hernandez-Delgadillo, Rene

AU - Zaragoza-Magaña, Valentin

AU - Sánchez-Pérez, Yesennia

AU - Meester, Irene

AU - Nakagoshi-Cepeda, Sergio Eduardo

AU - Solís-Soto, Juan Manuel

AU - Nakagoshi-Cepeda, María Argelia Akemi

AU - Chellam, Shankararaman

AU - Cabral-Romero, Claudio

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The objective of this study was to compare the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine (CHX) on human squamous cell carcinoma. BisBAL NPs were synthesized by colloidal method and characterized by energy dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (EDS-SEM). The effect of BisBAL NPs and CHX on oral cancer cell line (CAL-27) and nontumor control cell human gingival fibroblasts (HGFs) was determined by MTT cell viability assay. The obtained results showed selective inhibition of CAL-27 cell growth by BisBAL nanoclusters. A 24 h exposition to 25 μM BisBAL NP decreased 91% of CAL-27 cell growth, while nontumor HGFs cells were unaffected by BisBAL NPs showing 90% of cell viability. In contrast, CHX kills both CAL-27 and HGFs with the same efficacy. 25 μM of CHX decreased 97% and 80% of tumor and nontumoral cell growth. BisBAL NP and CHX alter cell permeability suggesting that action mechanism may include loss of cell membrane integrity. Also, CHX and not BisBAL NP presented genotoxicity on genomic DNA of tumor cells. As conclusion, BisBAL NPs have a selective antitumor activity on human squamous cell carcinoma, unlike CHX which was cytotoxic for both tumoral and nontumoral control cells.

AB - The objective of this study was to compare the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine (CHX) on human squamous cell carcinoma. BisBAL NPs were synthesized by colloidal method and characterized by energy dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (EDS-SEM). The effect of BisBAL NPs and CHX on oral cancer cell line (CAL-27) and nontumor control cell human gingival fibroblasts (HGFs) was determined by MTT cell viability assay. The obtained results showed selective inhibition of CAL-27 cell growth by BisBAL nanoclusters. A 24 h exposition to 25 μM BisBAL NP decreased 91% of CAL-27 cell growth, while nontumor HGFs cells were unaffected by BisBAL NPs showing 90% of cell viability. In contrast, CHX kills both CAL-27 and HGFs with the same efficacy. 25 μM of CHX decreased 97% and 80% of tumor and nontumoral cell growth. BisBAL NP and CHX alter cell permeability suggesting that action mechanism may include loss of cell membrane integrity. Also, CHX and not BisBAL NP presented genotoxicity on genomic DNA of tumor cells. As conclusion, BisBAL NPs have a selective antitumor activity on human squamous cell carcinoma, unlike CHX which was cytotoxic for both tumoral and nontumoral control cells.

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