"cLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica

F. Gonzalez-Salazar, I. Meester, F. J. Guzmán De La Garza, L. H. De La Garza-Salinas, A. Sampayo-Reyes, J. N. Garza-Gonzalez, O. Monsivais-Diaz, B. A. Barba-Dávila, M. E. Hernández-García, J. Vargas-Villarreal

Research output: Contribution to journalArticle

Abstract

Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

Original languageEnglish
Article number2796516
Number of pages6
JournalJournal of Parasitology Research
Volume2018
DOIs
Publication statusPublished - 11 Jul 2018

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Trophozoites
Entamoeba histolytica
Culture Media
Liver Abscess
Growth
Phospholipases
Amebiasis
Mesocricetus
Virulence Factors
Mexico
Animal Models
Costs and Cost Analysis
Lung
Liver
Health

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Gonzalez-Salazar, F. ; Meester, I. ; Guzmán De La Garza, F. J. ; De La Garza-Salinas, L. H. ; Sampayo-Reyes, A. ; Garza-Gonzalez, J. N. ; Monsivais-Diaz, O. ; Barba-Dávila, B. A. ; Hernández-García, M. E. ; Vargas-Villarreal, J. / "cLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. In: Journal of Parasitology Research. 2018 ; Vol. 2018.
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Gonzalez-Salazar, F, Meester, I, Guzmán De La Garza, FJ, De La Garza-Salinas, LH, Sampayo-Reyes, A, Garza-Gonzalez, JN, Monsivais-Diaz, O, Barba-Dávila, BA, Hernández-García, ME & Vargas-Villarreal, J 2018, '"cLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica', Journal of Parasitology Research, vol. 2018, 2796516. https://doi.org/10.1155/2018/2796516

"cLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. / Gonzalez-Salazar, F.; Meester, I.; Guzmán De La Garza, F. J.; De La Garza-Salinas, L. H.; Sampayo-Reyes, A.; Garza-Gonzalez, J. N.; Monsivais-Diaz, O.; Barba-Dávila, B. A.; Hernández-García, M. E.; Vargas-Villarreal, J.

In: Journal of Parasitology Research, Vol. 2018, 2796516, 11.07.2018.

Research output: Contribution to journalArticle

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T1 - "cLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica

AU - Gonzalez-Salazar, F.

AU - Meester, I.

AU - Guzmán De La Garza, F. J.

AU - De La Garza-Salinas, L. H.

AU - Sampayo-Reyes, A.

AU - Garza-Gonzalez, J. N.

AU - Monsivais-Diaz, O.

AU - Barba-Dávila, B. A.

AU - Hernández-García, M. E.

AU - Vargas-Villarreal, J.

PY - 2018/7/11

Y1 - 2018/7/11

N2 - Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

AB - Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

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