Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico

Samantha Flores-Treviño, Rayo Morfín-Otero, Eduardo Rodríguez-Noriega, Esteban González-Díaz, Héctor Raúl Pérez-Gómez, Soraya Mendoza-Olazarán, Isaías Balderas-Rentería, Gloria María González, Elvira Garza-González

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2 Citations (Scopus)

Abstract

INTRODUCTION: The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. The aim of this study was to determine phenotypic resistance and gene mutations associated with MDR of clinical isolates of Mycobacterium tuberculosis from Guadalajara, Mexico.

METHODS: One hundred and five isolates were subjected to drug susceptibility testing to first line drugs using the proportion and Mycobacteria Growth Indicator Tube (MGIT) methods. Genes associated with isoniazid (inhA, katG, ahpC) and rifampicin (rpoB) resistance were analyzed by either pyrosequencing or PCR-RFLP.

RESULTS: Resistance to any drug was detected in 48.6% of isolates, of which 40% were isoniazid-resistant, 20% were rifampicin-resistant and 19% were MDR. Drug-resistant isolates had the following frequency of mutations in rpoB (48%), katG (14%), inhA (26%), ahpC (26%). Susceptible isolates also had a mutation in ahpC (29%).

CONCLUSIONS: This is the first analysis of mutations associated with MDR of M. tuberculosis in Guadalajara. Commonly reported mutations worldwide were found in rpoB, katG and inhA genes. Substitution C to T in position -15 of the ahpC gene may possibly be a polymorphism.

Original languageEnglish
Pages (from-to)181-5
Number of pages5
JournalEnfermedades Infecciosas y Microbiologia Clinica
Volume33
Issue number3
DOIs
Publication statusPublished - Mar 2015
Externally publishedYes

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Mexico
Mycobacterium tuberculosis
Drug Resistance
Multidrug-Resistant Tuberculosis
Mutation
Isoniazid
Rifampin
Mycobacterium
Pharmaceutical Preparations
Genes
Mutation Rate
Restriction Fragment Length Polymorphisms
Tuberculosis
Delivery of Health Care
Polymerase Chain Reaction
Growth

Bibliographical note

Copyright © 2013 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

Cite this

Flores-Treviño, Samantha ; Morfín-Otero, Rayo ; Rodríguez-Noriega, Eduardo ; González-Díaz, Esteban ; Pérez-Gómez, Héctor Raúl ; Mendoza-Olazarán, Soraya ; Balderas-Rentería, Isaías ; González, Gloria María ; Garza-González, Elvira. / Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico. In: Enfermedades Infecciosas y Microbiologia Clinica. 2015 ; Vol. 33, No. 3. pp. 181-5.
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title = "Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico",
abstract = "INTRODUCTION: The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. The aim of this study was to determine phenotypic resistance and gene mutations associated with MDR of clinical isolates of Mycobacterium tuberculosis from Guadalajara, Mexico.METHODS: One hundred and five isolates were subjected to drug susceptibility testing to first line drugs using the proportion and Mycobacteria Growth Indicator Tube (MGIT) methods. Genes associated with isoniazid (inhA, katG, ahpC) and rifampicin (rpoB) resistance were analyzed by either pyrosequencing or PCR-RFLP.RESULTS: Resistance to any drug was detected in 48.6{\%} of isolates, of which 40{\%} were isoniazid-resistant, 20{\%} were rifampicin-resistant and 19{\%} were MDR. Drug-resistant isolates had the following frequency of mutations in rpoB (48{\%}), katG (14{\%}), inhA (26{\%}), ahpC (26{\%}). Susceptible isolates also had a mutation in ahpC (29{\%}).CONCLUSIONS: This is the first analysis of mutations associated with MDR of M. tuberculosis in Guadalajara. Commonly reported mutations worldwide were found in rpoB, katG and inhA genes. Substitution C to T in position -15 of the ahpC gene may possibly be a polymorphism.",
author = "Samantha Flores-Trevi{\~n}o and Rayo Morf{\'i}n-Otero and Eduardo Rodr{\'i}guez-Noriega and Esteban Gonz{\'a}lez-D{\'i}az and P{\'e}rez-G{\'o}mez, {H{\'e}ctor Ra{\'u}l} and Soraya Mendoza-Olazar{\'a}n and Isa{\'i}as Balderas-Renter{\'i}a and Gonz{\'a}lez, {Gloria Mar{\'i}a} and Elvira Garza-Gonz{\'a}lez",
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Flores-Treviño, S, Morfín-Otero, R, Rodríguez-Noriega, E, González-Díaz, E, Pérez-Gómez, HR, Mendoza-Olazarán, S, Balderas-Rentería, I, González, GM & Garza-González, E 2015, 'Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico', Enfermedades Infecciosas y Microbiologia Clinica, vol. 33, no. 3, pp. 181-5. https://doi.org/10.1016/j.eimc.2014.04.005

Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico. / Flores-Treviño, Samantha; Morfín-Otero, Rayo; Rodríguez-Noriega, Eduardo; González-Díaz, Esteban; Pérez-Gómez, Héctor Raúl; Mendoza-Olazarán, Soraya; Balderas-Rentería, Isaías; González, Gloria María; Garza-González, Elvira.

In: Enfermedades Infecciosas y Microbiologia Clinica, Vol. 33, No. 3, 03.2015, p. 181-5.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico

AU - Flores-Treviño, Samantha

AU - Morfín-Otero, Rayo

AU - Rodríguez-Noriega, Eduardo

AU - González-Díaz, Esteban

AU - Pérez-Gómez, Héctor Raúl

AU - Mendoza-Olazarán, Soraya

AU - Balderas-Rentería, Isaías

AU - González, Gloria María

AU - Garza-González, Elvira

N1 - Copyright © 2013 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

PY - 2015/3

Y1 - 2015/3

N2 - INTRODUCTION: The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. The aim of this study was to determine phenotypic resistance and gene mutations associated with MDR of clinical isolates of Mycobacterium tuberculosis from Guadalajara, Mexico.METHODS: One hundred and five isolates were subjected to drug susceptibility testing to first line drugs using the proportion and Mycobacteria Growth Indicator Tube (MGIT) methods. Genes associated with isoniazid (inhA, katG, ahpC) and rifampicin (rpoB) resistance were analyzed by either pyrosequencing or PCR-RFLP.RESULTS: Resistance to any drug was detected in 48.6% of isolates, of which 40% were isoniazid-resistant, 20% were rifampicin-resistant and 19% were MDR. Drug-resistant isolates had the following frequency of mutations in rpoB (48%), katG (14%), inhA (26%), ahpC (26%). Susceptible isolates also had a mutation in ahpC (29%).CONCLUSIONS: This is the first analysis of mutations associated with MDR of M. tuberculosis in Guadalajara. Commonly reported mutations worldwide were found in rpoB, katG and inhA genes. Substitution C to T in position -15 of the ahpC gene may possibly be a polymorphism.

AB - INTRODUCTION: The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. The aim of this study was to determine phenotypic resistance and gene mutations associated with MDR of clinical isolates of Mycobacterium tuberculosis from Guadalajara, Mexico.METHODS: One hundred and five isolates were subjected to drug susceptibility testing to first line drugs using the proportion and Mycobacteria Growth Indicator Tube (MGIT) methods. Genes associated with isoniazid (inhA, katG, ahpC) and rifampicin (rpoB) resistance were analyzed by either pyrosequencing or PCR-RFLP.RESULTS: Resistance to any drug was detected in 48.6% of isolates, of which 40% were isoniazid-resistant, 20% were rifampicin-resistant and 19% were MDR. Drug-resistant isolates had the following frequency of mutations in rpoB (48%), katG (14%), inhA (26%), ahpC (26%). Susceptible isolates also had a mutation in ahpC (29%).CONCLUSIONS: This is the first analysis of mutations associated with MDR of M. tuberculosis in Guadalajara. Commonly reported mutations worldwide were found in rpoB, katG and inhA genes. Substitution C to T in position -15 of the ahpC gene may possibly be a polymorphism.

U2 - 10.1016/j.eimc.2014.04.005

DO - 10.1016/j.eimc.2014.04.005

M3 - Article

VL - 33

SP - 181

EP - 185

JO - Enfermedades Infecciosas y Microbiologia Clinica

JF - Enfermedades Infecciosas y Microbiologia Clinica

SN - 0213-005X

IS - 3

ER -