Characterization of a cDNA clone encoding molluscan insulin‐related peptide II of Lymnaea stagnalis

August B. SMIT, Wijnand P.M. GERAERTS, Irene MEESTER, Harm van HEERIKHUIZEN, Joos JOOSSE

Research output: Contribution to journalArticle

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Abstract

A cDNA clone encoding molluscan insulin‐related peptide (MIP) II was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP cDNA (renamed MIP‐I cDNA). The MIP‐II cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and A and B chains; however, in this case connected by two distinct C peptides, Cα and Cβ, instead of a single C peptide, a phenomenon which represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIP II and I differ remarkably in primary structure; in contrast, the Cα peptide domains are fully identical. MIP II has only limited sequence similarity with insulins and related peptides. Both MIP II and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I and II genes are expressed in a single type of neuron: the growth‐controlling neuroendocrine light green cells of the Lymnaea CNS. Copyright © 1991, Wiley Blackwell. All rights reserved
Original languageEnglish
Pages (from-to)699-703
Number of pages5
JournalEuropean Journal of Biochemistry
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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Lymnaea
Complementary DNA
Clone Cells
Peptides
C-Peptide
Neurology
Central Nervous System
Insulin
Insulins
Methyl Green
Snails
Protein Sorting Signals
Fresh Water
Gene Library
Neurons
Screening
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

SMIT, August B. ; GERAERTS, Wijnand P.M. ; MEESTER, Irene ; van HEERIKHUIZEN, Harm ; JOOSSE, Joos. / Characterization of a cDNA clone encoding molluscan insulin‐related peptide II of Lymnaea stagnalis. In: European Journal of Biochemistry. 1991 ; pp. 699-703.
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abstract = "A cDNA clone encoding molluscan insulin‐related peptide (MIP) II was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP cDNA (renamed MIP‐I cDNA). The MIP‐II cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and A and B chains; however, in this case connected by two distinct C peptides, Cα and Cβ, instead of a single C peptide, a phenomenon which represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIP II and I differ remarkably in primary structure; in contrast, the Cα peptide domains are fully identical. MIP II has only limited sequence similarity with insulins and related peptides. Both MIP II and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I and II genes are expressed in a single type of neuron: the growth‐controlling neuroendocrine light green cells of the Lymnaea CNS. Copyright {\circledC} 1991, Wiley Blackwell. All rights reserved",
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Characterization of a cDNA clone encoding molluscan insulin‐related peptide II of Lymnaea stagnalis. / SMIT, August B.; GERAERTS, Wijnand P.M.; MEESTER, Irene; van HEERIKHUIZEN, Harm; JOOSSE, Joos.

In: European Journal of Biochemistry, 01.01.1991, p. 699-703.

Research output: Contribution to journalArticle

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