A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnae stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chai, however, in this case connected by two distinct C peptides, Cα and Cβ, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the Cα peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron: the growth controlling neuroendocrine light green cells of the Lymnaea CNS. © 1992.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cellular and Molecular Neuroscience
Smit, A. B., Thijsen, S. F. T., Geraerts, W. P. M., Meester, I., Van Heerikhuizen, H., & Joosse, J. (1992). Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis. Molecular Brain Research, 7-12. https://doi.org/10.1016/0169-328X(92)90003-T