Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis

August B. Smit, Steven F.T. Thijsen, Wijnand P.M. Geraerts, Irene Meester, Harm Van Heerikhuizen, Joos Joosse

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnae stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chai, however, in this case connected by two distinct C peptides, Cα and Cβ, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the Cα peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron: the growth controlling neuroendocrine light green cells of the Lymnaea CNS. © 1992.
Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalMolecular Brain Research
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

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Lymnaea
Complementary DNA
Clone Cells
Insulin
C-Peptide
Central Nervous System
Insulins
Methyl Green
Peptides
Snails
Protein Sorting Signals
Fresh Water
Gene Library
peptide V
Neurons
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Smit, August B. ; Thijsen, Steven F.T. ; Geraerts, Wijnand P.M. ; Meester, Irene ; Van Heerikhuizen, Harm ; Joosse, Joos. / Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis. In: Molecular Brain Research. 1992 ; pp. 7-12.
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abstract = "A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnae stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chai, however, in this case connected by two distinct C peptides, Cα and Cβ, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the Cα peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron: the growth controlling neuroendocrine light green cells of the Lymnaea CNS. {\circledC} 1992.",
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Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis. / Smit, August B.; Thijsen, Steven F.T.; Geraerts, Wijnand P.M.; Meester, Irene; Van Heerikhuizen, Harm; Joosse, Joos.

In: Molecular Brain Research, 01.01.1992, p. 7-12.

Research output: Contribution to journalArticle

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