Biochemical impact of solar radiation exposure on human keratinocytes monitored by Raman spectroscopy; effects of cell culture environment

Ulises Lopez-Gonzalez*, Alan Casey, Hugh J. Byrne

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding and amelioration of the effects of solar radiation exposure are critical in preventing the occurrence of skin cancer. Towards this end, many studies have been conducted in 2D cell culture models under simplified and unrealistic conditions. 3D culture models better capture the complexity of in vivo physiology, although the effects of the 3D extracellular matrix have not been well studied. Monitoring the instantaneous and resultant cellular responses to exposure, and the influence of the 3D environment, could provide an enhanced understanding of the fundamental processes of photocarcinogenesis. This work presents an analysis of the biochemical impacts of simulated solar radiation (SSR) occurring in immortalised human epithelial keratinocytes (HaCaT), in a 3D skin model, compared to 2D culture. Cell viability was monitored using the Alamar Blue colorimetric assay (AB), and the impact of the radiation exposure, at the level of the biomolecular constituents (nucleic acids and proteins), were evaluated through the combination of Raman microspectroscopy and multivariate statistical analysis. The results suggest that SSR exposure induces alterations of the conformational structure of DNA as an immediate impact, whereas changes in the protein signature are primarily seen as a subsequent response.

Original languageEnglish
Article numbere202100058
Pages (from-to)e202100058
JournalJournal of Biophotonics
Volume14
Issue number7
DOIs
Publication statusPublished - Jul 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy

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