Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium

Benito David Mata-Cárdenas, Magda Elizabeth Hernández-García, Francisco González-Salazar, Jesús Norberto Garza-González, Rebeca Palacios-Corona, Elva I. Cortés-Gutiérrez, Javier Vargas-Villarreal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum. © 2012 Versita Warsaw and Springer-Verlag Wien.
Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalActa Parasitologica
DOIs
Publication statusPublished - 1 Sep 2012
Externally publishedYes

Fingerprint

Giardia lamblia
Phospholipases A2
Serum-Free Culture Media
Serum
Culture Media
Giardia
Trichomonas vaginalis
Trophozoites
Entamoeba histolytica
Diamond
Specific Gravity
Polysorbates
Growth
Vitamins
Cholesterol
Lipids
Amino Acids

All Science Journal Classification (ASJC) codes

  • Parasitology

Cite this

Mata-Cárdenas, B. D., Hernández-García, M. E., González-Salazar, F., Garza-González, J. N., Palacios-Corona, R., Cortés-Gutiérrez, E. I., & Vargas-Villarreal, J. (2012). Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium. Acta Parasitologica, 211-215. https://doi.org/10.2478/s11686-012-0035-4
Mata-Cárdenas, Benito David ; Hernández-García, Magda Elizabeth ; González-Salazar, Francisco ; Garza-González, Jesús Norberto ; Palacios-Corona, Rebeca ; Cortés-Gutiérrez, Elva I. ; Vargas-Villarreal, Javier. / Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium. In: Acta Parasitologica. 2012 ; pp. 211-215.
@article{24e2909259d0435cb64561757bcb3bd3,
title = "Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium",
abstract = "Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95{\%} compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum. {\circledC} 2012 Versita Warsaw and Springer-Verlag Wien.",
author = "Mata-C{\'a}rdenas, {Benito David} and Hern{\'a}ndez-Garc{\'i}a, {Magda Elizabeth} and Francisco Gonz{\'a}lez-Salazar and Garza-Gonz{\'a}lez, {Jes{\'u}s Norberto} and Rebeca Palacios-Corona and Cort{\'e}s-Guti{\'e}rrez, {Elva I.} and Javier Vargas-Villarreal",
year = "2012",
month = "9",
day = "1",
doi = "10.2478/s11686-012-0035-4",
language = "English",
pages = "211--215",
journal = "Acta Parasitologica",
issn = "1230-2821",
publisher = "Walter de Gruyter GmbH",

}

Mata-Cárdenas, BD, Hernández-García, ME, González-Salazar, F, Garza-González, JN, Palacios-Corona, R, Cortés-Gutiérrez, EI & Vargas-Villarreal, J 2012, 'Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium', Acta Parasitologica, pp. 211-215. https://doi.org/10.2478/s11686-012-0035-4

Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium. / Mata-Cárdenas, Benito David; Hernández-García, Magda Elizabeth; González-Salazar, Francisco; Garza-González, Jesús Norberto; Palacios-Corona, Rebeca; Cortés-Gutiérrez, Elva I.; Vargas-Villarreal, Javier.

In: Acta Parasitologica, 01.09.2012, p. 211-215.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

AU - Mata-Cárdenas, Benito David

AU - Hernández-García, Magda Elizabeth

AU - González-Salazar, Francisco

AU - Garza-González, Jesús Norberto

AU - Palacios-Corona, Rebeca

AU - Cortés-Gutiérrez, Elva I.

AU - Vargas-Villarreal, Javier

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum. © 2012 Versita Warsaw and Springer-Verlag Wien.

AB - Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum. © 2012 Versita Warsaw and Springer-Verlag Wien.

U2 - 10.2478/s11686-012-0035-4

DO - 10.2478/s11686-012-0035-4

M3 - Article

SP - 211

EP - 215

JO - Acta Parasitologica

JF - Acta Parasitologica

SN - 1230-2821

ER -

Mata-Cárdenas BD, Hernández-García ME, González-Salazar F, Garza-González JN, Palacios-Corona R, Cortés-Gutiérrez EI et al. Axenic cultivation and comparative phospholipase A<inf>2</inf> activity of Giardia duodenalis in a serum-free medium. Acta Parasitologica. 2012 Sep 1;211-215. https://doi.org/10.2478/s11686-012-0035-4