Background: Mexican children are characterized by a high-starch intake diet and high prevalence of obesity. Objectives: To investigate the association of AMY1A/AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods: Anthropometric and dietary starch intake data were collected. CN of AMY1A/AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta-analysis of association between AMY1A CNVs and obesity was also performed. Results: A positive association between AMY1A/AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch (Pinteraction =.004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta-analysis of 3100 Mexican children. Conclusions: Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low-starch diet.
Bibliographical noteFunding Information:
Canada Research Chairs; Consejo Nacional de Ciencia y Tecnología; Instituto Mexicano del Seguro Social; Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México; Mexican Institute of Social Security under the program of Priority Health Topics 2017 Funding information
M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely Méndez‐Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola León‐Mimila and Pr. Samuel Canizales‐Quinteros for assistance in retrieving data for the meta‐analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México) and DLM (Ciencias Biomédicas PhD program from Universidad Autónoma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnología (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity.
© 2020 World Obesity Federation
Copyright 2020 Elsevier B.V., All rights reserved.
All Science Journal Classification (ASJC) codes
- Pediatrics, Perinatology, and Child Health
- Health Policy
- Nutrition and Dietetics
- Public Health, Environmental and Occupational Health