Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children

National Obesity Network Mexico; Francisco González-Salazar

doi:10.1111/ijpo.12641

Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children

National Obesity Network Mexico, Francisco González-Salazar

Departamento de Ciencias Básicas

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: Mexican children are characterized by a high-starch intake diet and high prevalence of obesity. Objectives: To investigate the association of AMY1A/AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods: Anthropometric and dietary starch intake data were collected. CN of AMY1A/AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta-analysis of association between AMY1A CNVs and obesity was also performed. Results: A positive association between AMY1A/AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch (P_interaction =.004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta-analysis of 3100 Mexican children. Conclusions: Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low-starch diet.

Original language	English
Article number	e12641
Journal	Pediatric obesity
Volume	15
Issue number	8
DOIs	https://doi.org/10.1111/ijpo.12641
Publication status	Published - 1 Aug 2020

Bibliographical note

Funding Information:
Canada Research Chairs; Consejo Nacional de Ciencia y Tecnología; Instituto Mexicano del Seguro Social; Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México; Mexican Institute of Social Security under the program of Priority Health Topics 2017 Funding information

Funding Information:
M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely Méndez‐Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola León‐Mimila and Pr. Samuel Canizales‐Quinteros for assistance in retrieving data for the meta‐analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México) and DLM (Ciencias Biomédicas PhD program from Universidad Autónoma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnología (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity.

Funding Information:
M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely M?ndez-Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola Le?n-Mimila and Pr. Samuel Canizales-Quinteros for assistance in retrieving data for the meta-analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias M?dicas Odontol?gicas y de la Salud PhD program from Universidad Nacional Aut?noma de M?xico) and DLM (Ciencias Biom?dicas PhD program from Universidad Aut?noma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnolog?a (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity.

Publisher Copyright:
© 2020 World Obesity Federation

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

All Science Journal Classification (ASJC) codes

Pediatrics, Perinatology, and Child Health
Health Policy
Nutrition and Dietetics
Public Health, Environmental and Occupational Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/ijpo.12641

Cite this

@article{302c37d8ca9c40b4a5062c8d142aadda,

title = "Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children",

abstract = "Background: Mexican children are characterized by a high-starch intake diet and high prevalence of obesity. Objectives: To investigate the association of AMY1A/AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods: Anthropometric and dietary starch intake data were collected. CN of AMY1A/AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta-analysis of association between AMY1A CNVs and obesity was also performed. Results: A positive association between AMY1A/AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch (Pinteraction =.004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta-analysis of 3100 Mexican children. Conclusions: Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low-starch diet.",

author = "{National Obesity Network Mexico} and Miguel V{\'a}zquez-Moreno and Aurora Mej{\'i}a-Ben{\'i}tez and Tanmay Sharma and Jes{\'u}s Peralta-Romero and Daniel Locia-Morales and Miguel Kl{\"u}nder-Kl{\"u}nder and {\'A}lvarez-Villase{\~n}or, {Andrea S.} and Acosta, {Kelly G.} and Raquel Flores-Torrecillas and Uriel Flores-Osuna and Garc{\'i}a-Avil{\'e}s, {Mariell G.} and {del Gonz{\'a}lez-Dzib}, {Roxana S.} and Gameros-Gardea, {Ren{\'e} A.} and Pizano-Z{\'a}rate, {Mar{\'i}a L.} and {del Romero-Figueroa}, {Mar{\'i}a S.} and N{\'u}{\~n}ez-Hern{\'a}ndez, {Jorge A.} and {de Le{\'o}n-Camacho}, Ver{\'o}nica and Roberto Karam-Araujo and Perla Corona-Salazar and Ad{\'a}n Valladares-Salgado and Fernando Suarez-S{\'a}nchez and Jaime G{\'o}mez-Zamudio and Eugenia Flores-Alfaro and Sosa-Bustamante, {Gloria P.} and Arturo Reyes-Hern{\'a}ndez and Catalina Peralta-Cort{\'a}zar and Mart{\'i}nez-Morales, {Emmanuel G.} and {D{\'i}az de Le{\'o}n-Morales}, {Luz V.} and {del Gonz{\'a}lez-Gonz{\'a}lez}, {Irma L.C.} and Reyes-Sosa, {Arturo M.} and Sonia Lazcano-Bautista and Marcela Mart{\'i}nez-Ch{\'a}vez and Gonz{\'a}lez-Serrano, {F{\'a}tima D.} and Rodr{\'i}guez-Alfaro, {Alma N.} and Murillo-Ortiz, {Blanca O.} and Arteaga-Alcaraz, {Mar{\'i}a G.} and Nandy Garc{\'i}a-Silva and Mois{\'e}s Herrera-Lemus and G{\'o}mez-Arteaga, {Gress M.} and Anel G{\'o}mez-Garc{\'i}a and Urbina-Trevi{\~n}o, {Martha V.} and Villalpando-S{\'a}nchez, {Diana C.} and Cleto {\'A}lvarez-Aguilar and Jim{\'e}nez-Arredondo, {Ram{\'o}n E.} and D{\'a}vila-Rodr{\'i}guez, {Martha I.} and Francisco Gonz{\'a}lez-Salazar and {de la Garza-Salinas}, {Laura H.} and Aleyda P{\'e}rez-Herrera and Socorro M{\'e}ndez-Mart{\'i}nez and Jorge Mart{\'i}nez-Torres",

note = "Funding Information: Canada Research Chairs; Consejo Nacional de Ciencia y Tecnolog{\'i}a; Instituto Mexicano del Seguro Social; Ciencias M{\'e}dicas Odontol{\'o}gicas y de la Salud PhD program from Universidad Nacional Aut{\'o}noma de M{\'e}xico; Mexican Institute of Social Security under the program of Priority Health Topics 2017 Funding information Funding Information: M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely M{\'e}ndez‐Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola Le{\'o}n‐Mimila and Pr. Samuel Canizales‐Quinteros for assistance in retrieving data for the meta‐analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias M{\'e}dicas Odontol{\'o}gicas y de la Salud PhD program from Universidad Nacional Aut{\'o}noma de M{\'e}xico) and DLM (Ciencias Biom{\'e}dicas PhD program from Universidad Aut{\'o}noma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity. Funding Information: M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely M?ndez-Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola Le?n-Mimila and Pr. Samuel Canizales-Quinteros for assistance in retrieving data for the meta-analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias M?dicas Odontol?gicas y de la Salud PhD program from Universidad Nacional Aut?noma de M?xico) and DLM (Ciencias Biom?dicas PhD program from Universidad Aut?noma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnolog?a (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity. Publisher Copyright: {\textcopyright} 2020 World Obesity Federation Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = aug,

day = "1",

doi = "10.1111/ijpo.12641",

language = "English",

volume = "15",

journal = "Pediatric obesity",

issn = "2047-6302",

publisher = "Wiley-Blackwell for the International Association for the Study of Obesity",

number = "8",

}

TY - JOUR

T1 - Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children

AU - National Obesity Network Mexico

AU - Vázquez-Moreno, Miguel

AU - Mejía-Benítez, Aurora

AU - Sharma, Tanmay

AU - Peralta-Romero, Jesús

AU - Locia-Morales, Daniel

AU - Klünder-Klünder, Miguel

AU - Álvarez-Villaseñor, Andrea S.

AU - Acosta, Kelly G.

AU - Flores-Torrecillas, Raquel

AU - Flores-Osuna, Uriel

AU - García-Avilés, Mariell G.

AU - del González-Dzib, Roxana S.

AU - Gameros-Gardea, René A.

AU - Pizano-Zárate, María L.

AU - del Romero-Figueroa, María S.

AU - Núñez-Hernández, Jorge A.

AU - de León-Camacho, Verónica

AU - Karam-Araujo, Roberto

AU - Corona-Salazar, Perla

AU - Valladares-Salgado, Adán

AU - Suarez-Sánchez, Fernando

AU - Gómez-Zamudio, Jaime

AU - Flores-Alfaro, Eugenia

AU - Sosa-Bustamante, Gloria P.

AU - Reyes-Hernández, Arturo

AU - Peralta-Cortázar, Catalina

AU - Martínez-Morales, Emmanuel G.

AU - Díaz de León-Morales, Luz V.

AU - del González-González, Irma L.C.

AU - Reyes-Sosa, Arturo M.

AU - Lazcano-Bautista, Sonia

AU - Martínez-Chávez, Marcela

AU - González-Serrano, Fátima D.

AU - Rodríguez-Alfaro, Alma N.

AU - Murillo-Ortiz, Blanca O.

AU - Arteaga-Alcaraz, María G.

AU - García-Silva, Nandy

AU - Herrera-Lemus, Moisés

AU - Gómez-Arteaga, Gress M.

AU - Gómez-García, Anel

AU - Urbina-Treviño, Martha V.

AU - Villalpando-Sánchez, Diana C.

AU - Álvarez-Aguilar, Cleto

AU - Jiménez-Arredondo, Ramón E.

AU - Dávila-Rodríguez, Martha I.

AU - González-Salazar, Francisco

AU - de la Garza-Salinas, Laura H.

AU - Pérez-Herrera, Aleyda

AU - Méndez-Martínez, Socorro

AU - Martínez-Torres, Jorge

N1 - Funding Information: Canada Research Chairs; Consejo Nacional de Ciencia y Tecnología; Instituto Mexicano del Seguro Social; Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México; Mexican Institute of Social Security under the program of Priority Health Topics 2017 Funding information Funding Information: M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely Méndez‐Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola León‐Mimila and Pr. Samuel Canizales‐Quinteros for assistance in retrieving data for the meta‐analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias Médicas Odontológicas y de la Salud PhD program from Universidad Nacional Autónoma de México) and DLM (Ciencias Biomédicas PhD program from Universidad Autónoma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnología (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity. Funding Information: M.V.M, M.C. and D.M. designed the study; M.V.M, A.M.B., T.S., J.P.R., D.L.M., M.K.K., M.C. and D.M. conducted research; M.V.M, A.M.B. and D.M. analyzed data; M.V.M., A.M.B. and D.M. wrote the manuscript; M.V.M., A.M.B. and D.M. designed the tables and figures. T.S., J.P.R., D.L.M., M.K.K. and M.C. critically reviewed the manuscript for important intellectual content; M.V.M, M.C. and D.M. had primary responsibility for final content. All authors read and approved the final manuscript. We are grateful to all participants involved in this study. We would like to acknowledge Aracely M?ndez-Padron (Instituto Mexicano del Seguro Social [IMSS], Mexico) and Anila Qasim (McMaster University, Canada) for the technical assistance. We would like to thank Dr. Paola Le?n-Mimila and Pr. Samuel Canizales-Quinteros for assistance in retrieving data for the meta-analysis. We would like to thank Dr. Jennifer Stearns for helpful discussions. This work was supported by grants from the Mexican Institute of Social Security under the program of Priority Health Topics 2017 (Grant No. FIS/IMSS/PROT/PRIO/17/062). MVM (Ciencias M?dicas Odontol?gicas y de la Salud PhD program from Universidad Nacional Aut?noma de M?xico) and DLM (Ciencias Biom?dicas PhD program from Universidad Aut?noma de Guerrero) were supported by PhD fellowships from the Consejo Nacional de Ciencia y Tecnolog?a (CONACYT) and IMSS (Mexico). In addition, MVM received a travel award from the IMSS, Mexico. D.M. is supported by a Canada Research Chair in Genetics of Obesity. Publisher Copyright: © 2020 World Obesity Federation Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Background: Mexican children are characterized by a high-starch intake diet and high prevalence of obesity. Objectives: To investigate the association of AMY1A/AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods: Anthropometric and dietary starch intake data were collected. CN of AMY1A/AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta-analysis of association between AMY1A CNVs and obesity was also performed. Results: A positive association between AMY1A/AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch (Pinteraction =.004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta-analysis of 3100 Mexican children. Conclusions: Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low-starch diet.

AB - Background: Mexican children are characterized by a high-starch intake diet and high prevalence of obesity. Objectives: To investigate the association of AMY1A/AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods: Anthropometric and dietary starch intake data were collected. CN of AMY1A/AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta-analysis of association between AMY1A CNVs and obesity was also performed. Results: A positive association between AMY1A/AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch (Pinteraction =.004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta-analysis of 3100 Mexican children. Conclusions: Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low-starch diet.

UR - http://www.scopus.com/inward/record.url?scp=85083716433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85083716433&partnerID=8YFLogxK

U2 - 10.1111/ijpo.12641

DO - 10.1111/ijpo.12641

M3 - Article

AN - SCOPUS:85083716433

SN - 2047-6302

VL - 15

JO - Pediatric obesity

JF - Pediatric obesity

IS - 8

M1 - e12641

ER -

Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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