Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858c/T gene polymorphisms in the risk of obesity in adolescents

Mauricio Andrés Salinas-Santander, Rafael Baltazar León-Cachón, Ana Cecilia Cepeda-Nieto, Celia Nohemí Sánchez-Domínguez, María Antonia González-Zavala, Hugo Leonid Gallardo-Blanco, Sandra Cecilia Esparza-González, Miguel Ángel González-Madrazo

Research output: Contribution to journalArticle

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Abstract

© 2015 Spandidos Publications. All rights reserved. Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.
Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalBiomedical Reports
Volume4
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

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Pediatric Obesity
Polymorphism
Tumor Necrosis Factor-alpha
Genes
Obesity
Insulin
Non-Receptor Type 22 Protein Tyrosine Phosphatase
Insulin Resistance
Genotype
Protein Tyrosine Phosphatases
Immune System Diseases
Chloroform
Phenol
Nutritional Status
Association reactions
Restriction Fragment Length Polymorphisms
Population
Autoimmune Diseases
Publications
Molecular Biology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Salinas-Santander, M. A., León-Cachón, R. B., Cepeda-Nieto, A. C., Sánchez-Domínguez, C. N., González-Zavala, M. A., Gallardo-Blanco, H. L., ... González-Madrazo, M. Á. (2016). Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858c/T gene polymorphisms in the risk of obesity in adolescents. Biomedical Reports, 4(1), 107-111. https://doi.org/10.3892/br.2015.534
Salinas-Santander, Mauricio Andrés ; León-Cachón, Rafael Baltazar ; Cepeda-Nieto, Ana Cecilia ; Sánchez-Domínguez, Celia Nohemí ; González-Zavala, María Antonia ; Gallardo-Blanco, Hugo Leonid ; Esparza-González, Sandra Cecilia ; González-Madrazo, Miguel Ángel. / Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858c/T gene polymorphisms in the risk of obesity in adolescents. In: Biomedical Reports. 2016 ; Vol. 4, No. 1. pp. 107-111.
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abstract = "{\circledC} 2015 Spandidos Publications. All rights reserved. Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.",
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Salinas-Santander, MA, León-Cachón, RB, Cepeda-Nieto, AC, Sánchez-Domínguez, CN, González-Zavala, MA, Gallardo-Blanco, HL, Esparza-González, SC & González-Madrazo, MÁ 2016, 'Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858c/T gene polymorphisms in the risk of obesity in adolescents', Biomedical Reports, vol. 4, no. 1, pp. 107-111. https://doi.org/10.3892/br.2015.534

Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858c/T gene polymorphisms in the risk of obesity in adolescents. / Salinas-Santander, Mauricio Andrés; León-Cachón, Rafael Baltazar; Cepeda-Nieto, Ana Cecilia; Sánchez-Domínguez, Celia Nohemí; González-Zavala, María Antonia; Gallardo-Blanco, Hugo Leonid; Esparza-González, Sandra Cecilia; González-Madrazo, Miguel Ángel.

In: Biomedical Reports, Vol. 4, No. 1, 01.01.2016, p. 107-111.

Research output: Contribution to journalArticle

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AU - León-Cachón, Rafael Baltazar

AU - Cepeda-Nieto, Ana Cecilia

AU - Sánchez-Domínguez, Celia Nohemí

AU - González-Zavala, María Antonia

AU - Gallardo-Blanco, Hugo Leonid

AU - Esparza-González, Sandra Cecilia

AU - González-Madrazo, Miguel Ángel

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Y1 - 2016/1/1

N2 - © 2015 Spandidos Publications. All rights reserved. Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.

AB - © 2015 Spandidos Publications. All rights reserved. Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.

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