Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms

Adriana Sampayo-Reyes, Alba Hernández, Naouale El-Yamani, Celsa López-Campos, Eduardo Mayet-Machado, Cuauhtémoc B. Rincón-Castañeda, María de L. Limones-Aguilar, Jesús E. López-Campos, Mario B. de león, Silvia González-Hernández, Diana Hinojosa-Garza, Ricardo Marcos

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Abstract

Inorganic arsenic (i-As) is an environmental carcinogen to which millions of people are chronically exposed mainly via drinking water. In this study, we used the comet assay to evaluate DNA damage in i-As-exposed inhabitants of the north of Mexico. The environmental monitoring and the exposure assessment were done by measuring both drinking water arsenic (As) content and total urinary As. In addition, the studied population was genetically characterized for four different glutathione S-transferase omega1 (GSTO1) polymorphisms (Ala140Asp, Glu155del, Glu208Lys, and Ala236Val) and the As (+3 oxidation state) methyltransferase (AS3MT) Met287Thr polymorphism to determine whether such variants influence As-related genotoxicity. As content in the drinking water of the population was found to range between 1 and 187 μg/l, with a mean concentration value of 16 μg/l. The total urinary As content of the exposed individuals was found to be correlated with the As content in drinking water, and subjects were classified as low (< 30 μg As/g creatinine), medium (31-60 μg As/g creatinine), and highly exposed (> 61 μg As/g creatinine). A positive association was found between the level of exposure and the genetic damage measured as percentage of DNA in tail (p < 0.001), and AS3MT Met287Thr was found to significantly influence the effect (p < 0.034) among children carrying the 287Thr variant allele. Altogether, our results evidenced that people living in As-contaminated areas are at risk and that AS3MT genetic variation may play an important role modulating such risk in northern Mexico, especially among children. © The Author 2010. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalToxicological Sciences
DOIs
Publication statusPublished - 13 Jun 2010
Externally publishedYes

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Arsenic
Glutathione Transferase
Polymorphism
DNA Damage
DNA
Drinking Water
Mexico
Environmental Carcinogens
Comet Assay
Environmental Monitoring
Environmental Exposure
Methyltransferases
Population
Assays
Creatinine
Alleles
Association reactions
Oxidation

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Sampayo-Reyes, A., Hernández, A., El-Yamani, N., López-Campos, C., Mayet-Machado, E., Rincón-Castañeda, C. B., ... Marcos, R. (2010). Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms. Toxicological Sciences, 63-71. https://doi.org/10.1093/toxsci/kfq173
Sampayo-Reyes, Adriana ; Hernández, Alba ; El-Yamani, Naouale ; López-Campos, Celsa ; Mayet-Machado, Eduardo ; Rincón-Castañeda, Cuauhtémoc B. ; Limones-Aguilar, María de L. ; López-Campos, Jesús E. ; de león, Mario B. ; González-Hernández, Silvia ; Hinojosa-Garza, Diana ; Marcos, Ricardo. / Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms. In: Toxicological Sciences. 2010 ; pp. 63-71.
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abstract = "Inorganic arsenic (i-As) is an environmental carcinogen to which millions of people are chronically exposed mainly via drinking water. In this study, we used the comet assay to evaluate DNA damage in i-As-exposed inhabitants of the north of Mexico. The environmental monitoring and the exposure assessment were done by measuring both drinking water arsenic (As) content and total urinary As. In addition, the studied population was genetically characterized for four different glutathione S-transferase omega1 (GSTO1) polymorphisms (Ala140Asp, Glu155del, Glu208Lys, and Ala236Val) and the As (+3 oxidation state) methyltransferase (AS3MT) Met287Thr polymorphism to determine whether such variants influence As-related genotoxicity. As content in the drinking water of the population was found to range between 1 and 187 μg/l, with a mean concentration value of 16 μg/l. The total urinary As content of the exposed individuals was found to be correlated with the As content in drinking water, and subjects were classified as low (< 30 μg As/g creatinine), medium (31-60 μg As/g creatinine), and highly exposed (> 61 μg As/g creatinine). A positive association was found between the level of exposure and the genetic damage measured as percentage of DNA in tail (p < 0.001), and AS3MT Met287Thr was found to significantly influence the effect (p < 0.034) among children carrying the 287Thr variant allele. Altogether, our results evidenced that people living in As-contaminated areas are at risk and that AS3MT genetic variation may play an important role modulating such risk in northern Mexico, especially among children. {\circledC} The Author 2010. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.",
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Sampayo-Reyes, A, Hernández, A, El-Yamani, N, López-Campos, C, Mayet-Machado, E, Rincón-Castañeda, CB, Limones-Aguilar, MDL, López-Campos, JE, de león, MB, González-Hernández, S, Hinojosa-Garza, D & Marcos, R 2010, 'Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms', Toxicological Sciences, pp. 63-71. https://doi.org/10.1093/toxsci/kfq173

Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms. / Sampayo-Reyes, Adriana; Hernández, Alba; El-Yamani, Naouale; López-Campos, Celsa; Mayet-Machado, Eduardo; Rincón-Castañeda, Cuauhtémoc B.; Limones-Aguilar, María de L.; López-Campos, Jesús E.; de león, Mario B.; González-Hernández, Silvia; Hinojosa-Garza, Diana; Marcos, Ricardo.

In: Toxicological Sciences, 13.06.2010, p. 63-71.

Research output: Contribution to journalArticle

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T1 - Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms

AU - Sampayo-Reyes, Adriana

AU - Hernández, Alba

AU - El-Yamani, Naouale

AU - López-Campos, Celsa

AU - Mayet-Machado, Eduardo

AU - Rincón-Castañeda, Cuauhtémoc B.

AU - Limones-Aguilar, María de L.

AU - López-Campos, Jesús E.

AU - de león, Mario B.

AU - González-Hernández, Silvia

AU - Hinojosa-Garza, Diana

AU - Marcos, Ricardo

PY - 2010/6/13

Y1 - 2010/6/13

N2 - Inorganic arsenic (i-As) is an environmental carcinogen to which millions of people are chronically exposed mainly via drinking water. In this study, we used the comet assay to evaluate DNA damage in i-As-exposed inhabitants of the north of Mexico. The environmental monitoring and the exposure assessment were done by measuring both drinking water arsenic (As) content and total urinary As. In addition, the studied population was genetically characterized for four different glutathione S-transferase omega1 (GSTO1) polymorphisms (Ala140Asp, Glu155del, Glu208Lys, and Ala236Val) and the As (+3 oxidation state) methyltransferase (AS3MT) Met287Thr polymorphism to determine whether such variants influence As-related genotoxicity. As content in the drinking water of the population was found to range between 1 and 187 μg/l, with a mean concentration value of 16 μg/l. The total urinary As content of the exposed individuals was found to be correlated with the As content in drinking water, and subjects were classified as low (< 30 μg As/g creatinine), medium (31-60 μg As/g creatinine), and highly exposed (> 61 μg As/g creatinine). A positive association was found between the level of exposure and the genetic damage measured as percentage of DNA in tail (p < 0.001), and AS3MT Met287Thr was found to significantly influence the effect (p < 0.034) among children carrying the 287Thr variant allele. Altogether, our results evidenced that people living in As-contaminated areas are at risk and that AS3MT genetic variation may play an important role modulating such risk in northern Mexico, especially among children. © The Author 2010. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.

AB - Inorganic arsenic (i-As) is an environmental carcinogen to which millions of people are chronically exposed mainly via drinking water. In this study, we used the comet assay to evaluate DNA damage in i-As-exposed inhabitants of the north of Mexico. The environmental monitoring and the exposure assessment were done by measuring both drinking water arsenic (As) content and total urinary As. In addition, the studied population was genetically characterized for four different glutathione S-transferase omega1 (GSTO1) polymorphisms (Ala140Asp, Glu155del, Glu208Lys, and Ala236Val) and the As (+3 oxidation state) methyltransferase (AS3MT) Met287Thr polymorphism to determine whether such variants influence As-related genotoxicity. As content in the drinking water of the population was found to range between 1 and 187 μg/l, with a mean concentration value of 16 μg/l. The total urinary As content of the exposed individuals was found to be correlated with the As content in drinking water, and subjects were classified as low (< 30 μg As/g creatinine), medium (31-60 μg As/g creatinine), and highly exposed (> 61 μg As/g creatinine). A positive association was found between the level of exposure and the genetic damage measured as percentage of DNA in tail (p < 0.001), and AS3MT Met287Thr was found to significantly influence the effect (p < 0.034) among children carrying the 287Thr variant allele. Altogether, our results evidenced that people living in As-contaminated areas are at risk and that AS3MT genetic variation may play an important role modulating such risk in northern Mexico, especially among children. © The Author 2010. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.

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DO - 10.1093/toxsci/kfq173

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JO - Toxicological Sciences

JF - Toxicological Sciences

SN - 1096-6080

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Sampayo-Reyes A, Hernández A, El-Yamani N, López-Campos C, Mayet-Machado E, Rincón-Castañeda CB et al. Arsenic induces DNA damage in environmentally exposed Mexican children and adults. Influence of GSTO1 and AS3MT Polymorphisms. Toxicological Sciences. 2010 Jun 13;63-71. https://doi.org/10.1093/toxsci/kfq173