Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions

Jesús Norberto Garza-González, Javier Vargas-Villarreal, Ma Julia Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cárdenas, Magda E. Hernandez-García, Laura De La Garza-Salinas, Francisco González-Salazar

Research output: Contribution to journalArticle

Abstract

Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic
growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts.
Next, the extract was fractionated by polarity-based partitioning. Then, the
purity of each fraction was determined by thin layer chromatography (TLC).
The percentage of growth inhibition was calculated with respect to untreated
controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the
aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of
G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited.
The methanol extract was further separated into mixtures of ursolic, oleanolic,
and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T.
vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The
methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites
Original languageUndefined/Unknown
Pages (from-to)1081-1094
Number of pages14
JournalHealth
Volume09
Issue number07
DOIs
Publication statusPublished - 2017

Cite this

Garza-González, J. N., Vargas-Villarreal, J., Verde-Star, M. J., Rivas-Morales, C., Oranday-Cárdenas, A., Hernandez-García, M. E., ... González-Salazar, F. (2017). Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions. Health, 09(07), 1081-1094. https://doi.org/10.4236/health.2017.97079
Garza-González, Jesús Norberto ; Vargas-Villarreal, Javier ; Verde-Star, Ma Julia ; Rivas-Morales, Catalina ; Oranday-Cárdenas, Azucena ; Hernandez-García, Magda E. ; Garza-Salinas, Laura De La ; González-Salazar, Francisco. / Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions. In: Health. 2017 ; Vol. 09, No. 07. pp. 1081-1094.
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title = "Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions",
abstract = "Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmicgrowth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts.Next, the extract was fractionated by polarity-based partitioning. Then, thepurity of each fraction was determined by thin layer chromatography (TLC).The percentage of growth inhibition was calculated with respect to untreatedcontrols. The 50{\%} inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of theaerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth ofG. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited.The methanol extract was further separated into mixtures of ursolic, oleanolic,and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T.vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: Themethanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites",
author = "Garza-Gonz{\'a}lez, {Jes{\'u}s Norberto} and Javier Vargas-Villarreal and Verde-Star, {Ma Julia} and Catalina Rivas-Morales and Azucena Oranday-C{\'a}rdenas and Hernandez-Garc{\'i}a, {Magda E.} and Garza-Salinas, {Laura De La} and Francisco Gonz{\'a}lez-Salazar",
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Garza-González, JN, Vargas-Villarreal, J, Verde-Star, MJ, Rivas-Morales, C, Oranday-Cárdenas, A, Hernandez-García, ME, Garza-Salinas, LDL & González-Salazar, F 2017, 'Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions', Health, vol. 09, no. 07, pp. 1081-1094. https://doi.org/10.4236/health.2017.97079

Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions. / Garza-González, Jesús Norberto; Vargas-Villarreal, Javier; Verde-Star, Ma Julia; Rivas-Morales, Catalina; Oranday-Cárdenas, Azucena; Hernandez-García, Magda E.; Garza-Salinas, Laura De La; González-Salazar, Francisco.

In: Health, Vol. 09, No. 07, 2017, p. 1081-1094.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions

AU - Garza-González, Jesús Norberto

AU - Vargas-Villarreal, Javier

AU - Verde-Star, Ma Julia

AU - Rivas-Morales, Catalina

AU - Oranday-Cárdenas, Azucena

AU - Hernandez-García, Magda E.

AU - Garza-Salinas, Laura De La

AU - González-Salazar, Francisco

PY - 2017

Y1 - 2017

N2 - Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmicgrowth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts.Next, the extract was fractionated by polarity-based partitioning. Then, thepurity of each fraction was determined by thin layer chromatography (TLC).The percentage of growth inhibition was calculated with respect to untreatedcontrols. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of theaerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth ofG. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited.The methanol extract was further separated into mixtures of ursolic, oleanolic,and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T.vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: Themethanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites

AB - Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmicgrowth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts.Next, the extract was fractionated by polarity-based partitioning. Then, thepurity of each fraction was determined by thin layer chromatography (TLC).The percentage of growth inhibition was calculated with respect to untreatedcontrols. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of theaerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth ofG. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited.The methanol extract was further separated into mixtures of ursolic, oleanolic,and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T.vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: Themethanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites

U2 - 10.4236/health.2017.97079

DO - 10.4236/health.2017.97079

M3 - Article

VL - 09

SP - 1081

EP - 1094

JO - Health (United Kingdom)

JF - Health (United Kingdom)

SN - 1363-4593

IS - 07

ER -

Garza-González JN, Vargas-Villarreal J, Verde-Star MJ, Rivas-Morales C, Oranday-Cárdenas A, Hernandez-García ME et al. Antiprotozoal Activity of a Thymus vulgaris Methanol Extract and Its Fractions. Health. 2017;09(07):1081-1094. https://doi.org/10.4236/health.2017.97079