Antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis

Ramiro Quintanilla-Licea, Benito David Mata-Cárdenas, Javier Vargas-Villarreal, Aldo Fabio Bazaldúa-Rodríguez, Isvar Kavim Ángeles-Hernández, Jesús Norberto Garza-González, Magda Elizabeth Hernández-García

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

© 2014 by the authors licensee MDPI Basel Switzerland. Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80% growth inhibition against E. histolytica at a concentration of 150 μg/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54% and 90.50% growth inhibition at a concentration of 150 μg/mL with IC50 values of 59.14 and 60.07 μg/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.
Original languageEnglish
Pages (from-to)21044-21065
Number of pages22
JournalMolecules
DOIs
Publication statusPublished - 1 Dec 2014
Externally publishedYes

Fingerprint

Lippia
Ruta
Entamoeba histolytica
Traditional Medicine
activity (biology)
medicine
Medicine
Antiprotozoal Agents
Plant Extracts
Protozoa
Antiparasitic Agents
Inhibitory Concentration 50
Bioassay
Metronidazole
Public health
Fractionation
Medical problems
Developing countries
Amebiasis
Trophozoites

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Quintanilla-Licea, R., Mata-Cárdenas, B. D., Vargas-Villarreal, J., Bazaldúa-Rodríguez, A. F., Ángeles-Hernández, I. K., Garza-González, J. N., & Hernández-García, M. E. (2014). Antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis. Molecules, 21044-21065. https://doi.org/10.3390/molecules191221044
Quintanilla-Licea, Ramiro ; Mata-Cárdenas, Benito David ; Vargas-Villarreal, Javier ; Bazaldúa-Rodríguez, Aldo Fabio ; Ángeles-Hernández, Isvar Kavim ; Garza-González, Jesús Norberto ; Hernández-García, Magda Elizabeth. / Antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis. In: Molecules. 2014 ; pp. 21044-21065.
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abstract = "{\circledC} 2014 by the authors licensee MDPI Basel Switzerland. Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80{\%} growth inhibition against E. histolytica at a concentration of 150 μg/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54{\%} and 90.50{\%} growth inhibition at a concentration of 150 μg/mL with IC50 values of 59.14 and 60.07 μg/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.",
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Quintanilla-Licea, R, Mata-Cárdenas, BD, Vargas-Villarreal, J, Bazaldúa-Rodríguez, AF, Ángeles-Hernández, IK, Garza-González, JN & Hernández-García, ME 2014, 'Antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis', Molecules, pp. 21044-21065. https://doi.org/10.3390/molecules191221044

Antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis. / Quintanilla-Licea, Ramiro; Mata-Cárdenas, Benito David; Vargas-Villarreal, Javier; Bazaldúa-Rodríguez, Aldo Fabio; Ángeles-Hernández, Isvar Kavim; Garza-González, Jesús Norberto; Hernández-García, Magda Elizabeth.

In: Molecules, 01.12.2014, p. 21044-21065.

Research output: Contribution to journalArticle

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AU - Quintanilla-Licea, Ramiro

AU - Mata-Cárdenas, Benito David

AU - Vargas-Villarreal, Javier

AU - Bazaldúa-Rodríguez, Aldo Fabio

AU - Ángeles-Hernández, Isvar Kavim

AU - Garza-González, Jesús Norberto

AU - Hernández-García, Magda Elizabeth

PY - 2014/12/1

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N2 - © 2014 by the authors licensee MDPI Basel Switzerland. Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80% growth inhibition against E. histolytica at a concentration of 150 μg/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54% and 90.50% growth inhibition at a concentration of 150 μg/mL with IC50 values of 59.14 and 60.07 μg/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.

AB - © 2014 by the authors licensee MDPI Basel Switzerland. Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80% growth inhibition against E. histolytica at a concentration of 150 μg/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54% and 90.50% growth inhibition at a concentration of 150 μg/mL with IC50 values of 59.14 and 60.07 μg/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.

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