Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

Jonathan Perez-Meseguer, Liliana Torres-González, Jorge Aurelio Gutiérrez-González, Gabriela Alarcón-Galván, Homero Zapata-Chavira, Noemi Waksman-De Torres, Diana Patricia Moreno-Peña, Linda Elsa Muñoz-Espinosa, Paula Cordero-Pérez

Research output: Contribution to journalArticle

Abstract

Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

Original languageEnglish
Article number186
JournalBMC Complementary and Alternative Medicine
Volume19
Issue number1
DOIs
Publication statusPublished - 26 Jul 2019

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Juglans
Reperfusion Injury
Wistar Rats
Anti-Inflammatory Agents
Ischemia
Kidney
Reperfusion
Malondialdehyde
Interleukin-1
Superoxide Dismutase
Interleukin-6
Alanine Transaminase
Creatinine
Oxidative Stress
Blood Urea Nitrogen
Constriction
Tumor Necrosis Factor-alpha
Antioxidants
Cytokines

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Perez-Meseguer, J., Torres-González, L., Gutiérrez-González, J. A., Alarcón-Galván, G., Zapata-Chavira, H., Waksman-De Torres, N., ... Cordero-Pérez, P. (2019). Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. BMC Complementary and Alternative Medicine, 19(1), [186]. https://doi.org/10.1186/s12906-019-2604-7
Perez-Meseguer, Jonathan ; Torres-González, Liliana ; Gutiérrez-González, Jorge Aurelio ; Alarcón-Galván, Gabriela ; Zapata-Chavira, Homero ; Waksman-De Torres, Noemi ; Moreno-Peña, Diana Patricia ; Muñoz-Espinosa, Linda Elsa ; Cordero-Pérez, Paula. / Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. In: BMC Complementary and Alternative Medicine. 2019 ; Vol. 19, No. 1.
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abstract = "Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.",
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Perez-Meseguer, J, Torres-González, L, Gutiérrez-González, JA, Alarcón-Galván, G, Zapata-Chavira, H, Waksman-De Torres, N, Moreno-Peña, DP, Muñoz-Espinosa, LE & Cordero-Pérez, P 2019, 'Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model', BMC Complementary and Alternative Medicine, vol. 19, no. 1, 186. https://doi.org/10.1186/s12906-019-2604-7

Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. / Perez-Meseguer, Jonathan; Torres-González, Liliana; Gutiérrez-González, Jorge Aurelio; Alarcón-Galván, Gabriela; Zapata-Chavira, Homero; Waksman-De Torres, Noemi; Moreno-Peña, Diana Patricia; Muñoz-Espinosa, Linda Elsa; Cordero-Pérez, Paula.

In: BMC Complementary and Alternative Medicine, Vol. 19, No. 1, 186, 26.07.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

AU - Perez-Meseguer, Jonathan

AU - Torres-González, Liliana

AU - Gutiérrez-González, Jorge Aurelio

AU - Alarcón-Galván, Gabriela

AU - Zapata-Chavira, Homero

AU - Waksman-De Torres, Noemi

AU - Moreno-Peña, Diana Patricia

AU - Muñoz-Espinosa, Linda Elsa

AU - Cordero-Pérez, Paula

PY - 2019/7/26

Y1 - 2019/7/26

N2 - Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

AB - Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

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