Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

Jonathan Perez-Meseguer, Liliana Torres-González, Jorge Aurelio Gutiérrez-González, Gabriela Alarcón-Galván, Homero Zapata-Chavira, Noemi Waksman-De Torres, Diana Patricia Moreno-Peña, Linda Elsa Muñoz-Espinosa, Paula Cordero-Pérez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

Original languageEnglish
Article number186
Pages (from-to)186
JournalBMC Complementary and Alternative Medicine
Issue number1
Publication statusPublished - 26 Jul 2019

Bibliographical note

Funding Information:
The authors gratefully acknowledge CONACYT Mexico for support through Red Farmoquimicos (CONACYT No 294727). Collection, analysis and writing of this project were financially supported by resources of the Liver Unit.

Publisher Copyright:
© 2019 The Author(s).

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine


Dive into the research topics of 'Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model'. Together they form a unique fingerprint.

Cite this