Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

Jonathan Perez-Meseguer; Liliana Torres-González; Jorge Aurelio Gutiérrez-González; Gabriela Alarcón-Galván; Homero Zapata-Chavira; Noemi Waksman-De Torres; Diana Patricia Moreno-Peña; Linda Elsa Muñoz-Espinosa; Paula Cordero-Pérez

doi:10.1186/s12906-019-2604-7

Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

Jonathan Perez-Meseguer, Liliana Torres-González, Jorge Aurelio Gutiérrez-González, Gabriela Alarcón-Galván, Homero Zapata-Chavira, Noemi Waksman-De Torres, Diana Patricia Moreno-Peña, Linda Elsa Muñoz-Espinosa, Paula Cordero-Pérez^*

^*Corresponding author for this work

Departamento de Ciencias Básicas

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

Original language	English
Article number	186
Pages (from-to)	186
Journal	BMC Complementary and Alternative Medicine
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12906-019-2604-7
Publication status	Published - 26 Jul 2019

Bibliographical note

Funding Information:
The authors gratefully acknowledge CONACYT Mexico for support through Red Farmoquimicos (CONACYT No 294727). Collection, analysis and writing of this project were financially supported by resources of the Liver Unit.

Publisher Copyright:
© 2019 The Author(s).

All Science Journal Classification (ASJC) codes

Complementary and alternative medicine

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10.1186/s12906-019-2604-7

Cite this

Perez-Meseguer, J., Torres-González, L., Gutiérrez-González, J. A., Alarcón-Galván, G., Zapata-Chavira, H., Waksman-De Torres, N., Moreno-Peña, D. P., Muñoz-Espinosa, L. E., & Cordero-Pérez, P. (2019). Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. BMC Complementary and Alternative Medicine, 19(1), 186. Article 186. https://doi.org/10.1186/s12906-019-2604-7

@article{f7e64521d0bb43b79399215e6d2cafb4,

title = "Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model",

abstract = "Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.",

author = "Jonathan Perez-Meseguer and Liliana Torres-Gonz{\'a}lez and Guti{\'e}rrez-Gonz{\'a}lez, {Jorge Aurelio} and Gabriela Alarc{\'o}n-Galv{\'a}n and Homero Zapata-Chavira and {Waksman-De Torres}, Noemi and Moreno-Pe{\~n}a, {Diana Patricia} and Mu{\~n}oz-Espinosa, {Linda Elsa} and Paula Cordero-P{\'e}rez",

note = "Funding Information: The authors gratefully acknowledge CONACYT Mexico for support through Red Farmoquimicos (CONACYT No 294727). Collection, analysis and writing of this project were financially supported by resources of the Liver Unit. Publisher Copyright: {\textcopyright} 2019 The Author(s).",

year = "2019",

month = jul,

day = "26",

doi = "10.1186/s12906-019-2604-7",

language = "English",

volume = "19",

pages = "186",

journal = "BMC Complementary and Alternative Medicine",

issn = "1472-6882",

publisher = "BioMed Central",

number = "1",

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Perez-Meseguer, J, Torres-González, L, Gutiérrez-González, JA, Alarcón-Galván, G, Zapata-Chavira, H, Waksman-De Torres, N, Moreno-Peña, DP, Muñoz-Espinosa, LE & Cordero-Pérez, P 2019, 'Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model', BMC Complementary and Alternative Medicine, vol. 19, no. 1, 186, pp. 186. https://doi.org/10.1186/s12906-019-2604-7

Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model. / Perez-Meseguer, Jonathan; Torres-González, Liliana; Gutiérrez-González, Jorge Aurelio et al.
In: BMC Complementary and Alternative Medicine, Vol. 19, No. 1, 186, 26.07.2019, p. 186.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

AU - Perez-Meseguer, Jonathan

AU - Torres-González, Liliana

AU - Gutiérrez-González, Jorge Aurelio

AU - Alarcón-Galván, Gabriela

AU - Zapata-Chavira, Homero

AU - Waksman-De Torres, Noemi

AU - Moreno-Peña, Diana Patricia

AU - Muñoz-Espinosa, Linda Elsa

AU - Cordero-Pérez, Paula

N1 - Funding Information: The authors gratefully acknowledge CONACYT Mexico for support through Red Farmoquimicos (CONACYT No 294727). Collection, analysis and writing of this project were financially supported by resources of the Liver Unit. Publisher Copyright: © 2019 The Author(s).

PY - 2019/7/26

Y1 - 2019/7/26

N2 - Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

AB - Background: Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury. Methods: Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α). Results: The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats. Conclusions: To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.

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U2 - 10.1186/s12906-019-2604-7

DO - 10.1186/s12906-019-2604-7

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SN - 1472-6882

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JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

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ER -

Anti-inflammatory and nephroprotective activity of Juglans mollis against renal ischemia-reperfusion damage in a Wistar rat model

Abstract

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