TY - JOUR
T1 - Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells
AU - Vargas-Villarreal, Javier
AU - Cruz-Ramos, Marlid
AU - Espino-Ojeda, Alba
AU - Gutierrez-Hermosillo, Hugo
AU - DE LEON-GONZALEZ, Enrique Díaz
AU - Monsivais-Diaz, Ofelia
AU - Palacios-Corona, Rebeca
AU - Martinez-Armenta, Carlos Alejandro
AU - González Salazar, Francisco
AU - Moreno-Treviño, Maria Guadalupe
AU - GUZMAN-DE LA GARZA, Francisco Javier
N1 - Funding Information:
The present study was supported by the Instituto Mexicano del Seguro Social (grant no. FIS/IMSS/PROT/G 2006/1A/I/080).
Publisher Copyright:
© 2021, Spandidos Publications. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Malignant ascites (MA) and malignant pleural effusion (MPE) are frequently developed in patients with metastatic cancer; however, the biological properties of these fluids have not been clarified. The present study explored the biological role of a low molecular fraction derived from malignant effusions on the activation of peripheral blood mononuclear cells and on the proliferation of breast cancer cells and fibroblast 55x cells. A <10-kDa fraction from effusions of 41 oncological patients and 34 individuals without cancer was purified, and its potential role in inhibiting nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells was explored, as well as its cytotoxicity on MCF-7 breast cancer cells and fibroblast 55x cells. A significant decrease in NO production was observed in the <10-kDa fraction from malignant effusions. In addition, the acellular fraction from MA decreased the viability of breast cancer cells without affecting human fibroblasts. These data support the presence of low molecular weight molecules in malignant samples with a specific role in inhibiting the defense mechanisms of peripheral blood mononuclear cells and decreasing the viability of breast cancer cells in vitro.
AB - Malignant ascites (MA) and malignant pleural effusion (MPE) are frequently developed in patients with metastatic cancer; however, the biological properties of these fluids have not been clarified. The present study explored the biological role of a low molecular fraction derived from malignant effusions on the activation of peripheral blood mononuclear cells and on the proliferation of breast cancer cells and fibroblast 55x cells. A <10-kDa fraction from effusions of 41 oncological patients and 34 individuals without cancer was purified, and its potential role in inhibiting nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells was explored, as well as its cytotoxicity on MCF-7 breast cancer cells and fibroblast 55x cells. A significant decrease in NO production was observed in the <10-kDa fraction from malignant effusions. In addition, the acellular fraction from MA decreased the viability of breast cancer cells without affecting human fibroblasts. These data support the presence of low molecular weight molecules in malignant samples with a specific role in inhibiting the defense mechanisms of peripheral blood mononuclear cells and decreasing the viability of breast cancer cells in vitro.
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U2 - 10.3892/mco.2021.2268
DO - 10.3892/mco.2021.2268
M3 - Article
C2 - 33796293
AN - SCOPUS:85103860663
SN - 2049-9450
VL - 14
JO - Molecular and Clinical Oncology
JF - Molecular and Clinical Oncology
IS - 5
M1 - 106
ER -
