A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy

Sandra K. Santuario-Facio, Servando Cardona-Huerta, Yadira X. Perez-Paramo, Victor Trevino, Francisco Hernandez-Cabrera, Augusto Rojas-Martinez, Grecia Uscanga-Perales, Jorge L. Martinez-Rodriguez, Lizeth Martinez-Jacobo, Gerardo Padilla-Rivas, Gerardo Muñoz-Maldonado, Juan Francisco Gonzalez-Guerrero, Javier Valero-Gomez, Ana L. Vazquez-Guerrero, Herminia G. Martinez-Rodriguez, Alvaro Barboza-Quintana, Oralia Barboza-Quintana, Raquel Garza-Guajardo, Rocio Ortiz-Lopez

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer tumors. Comparisons between TNBC and non-triple-negative breast cancer (nTNBC) may help to differentiate key components involved in TNBC neoplasms. The purpose of the study was to analyze the expression profile of TNBC versus nTNBC tumors in a homogeneous population from northeastern Mexico. A prospective study of 50 patients (25 TNBC and 25 nTNBC) was conducted. Clinic parameters were equally distributed for TNBC and nTNBC: age at diagnosis (51 versus 47 years, p = 0.1), glucose level (107 mg/dl versus 104 mg/dl, p = 0.64), and body mass index (28 versus 29, p = 0.14). Core biopsies were collected for histopathological diagnosis and gene expression analysis. Total RNA was isolated and expression profiling was performed. Forty genes showed differential expression pattern in TNBC tumors. Among these, nine overexpressed genes (PRKX/PRKY, UGT8, HMGA1, LPIN1, HAPLN3, FAM171A1, BCL141A, FOXC1, and ANKRD11), and one underexpressed gene (ANX9) are involved in general metabolism. Based on this biochemical peculiarity and the overexpression of BCL11A and FOXC1 (involved in tumor growth and metastasis, respectively), we validated by quantitative polymerase chain reaction the expression profiles of seven genes out of the signature. In this report, a new gene signature for TNBC is proposed. To our knowledge, this is the first TNBC signature that describes genes involved in general metabolism. The findings may be pertinent for Mexican patients and require evaluation in other ethnic groups and populations.

Original languageEnglish
Pages (from-to)101-111
Number of pages11
JournalMolecular Medicine
Volume23
DOIs
Publication statusPublished - Jun 2017
Externally publishedYes

Fingerprint

Triple Negative Breast Neoplasms
Transcriptome
Drug Therapy
Breast Neoplasms
Genes
Mexico
Ethnic Groups
Population
Body Mass Index
Prospective Studies
RNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Santuario-Facio, S. K., Cardona-Huerta, S., Perez-Paramo, Y. X., Trevino, V., Hernandez-Cabrera, F., Rojas-Martinez, A., ... Ortiz-Lopez, R. (2017). A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy. Molecular Medicine, 23, 101-111. https://doi.org/10.2119/molmed.2016.00257
Santuario-Facio, Sandra K. ; Cardona-Huerta, Servando ; Perez-Paramo, Yadira X. ; Trevino, Victor ; Hernandez-Cabrera, Francisco ; Rojas-Martinez, Augusto ; Uscanga-Perales, Grecia ; Martinez-Rodriguez, Jorge L. ; Martinez-Jacobo, Lizeth ; Padilla-Rivas, Gerardo ; Muñoz-Maldonado, Gerardo ; Gonzalez-Guerrero, Juan Francisco ; Valero-Gomez, Javier ; Vazquez-Guerrero, Ana L. ; Martinez-Rodriguez, Herminia G. ; Barboza-Quintana, Alvaro ; Barboza-Quintana, Oralia ; Garza-Guajardo, Raquel ; Ortiz-Lopez, Rocio. / A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy. In: Molecular Medicine. 2017 ; Vol. 23. pp. 101-111.
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abstract = "Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer tumors. Comparisons between TNBC and non-triple-negative breast cancer (nTNBC) may help to differentiate key components involved in TNBC neoplasms. The purpose of the study was to analyze the expression profile of TNBC versus nTNBC tumors in a homogeneous population from northeastern Mexico. A prospective study of 50 patients (25 TNBC and 25 nTNBC) was conducted. Clinic parameters were equally distributed for TNBC and nTNBC: age at diagnosis (51 versus 47 years, p = 0.1), glucose level (107 mg/dl versus 104 mg/dl, p = 0.64), and body mass index (28 versus 29, p = 0.14). Core biopsies were collected for histopathological diagnosis and gene expression analysis. Total RNA was isolated and expression profiling was performed. Forty genes showed differential expression pattern in TNBC tumors. Among these, nine overexpressed genes (PRKX/PRKY, UGT8, HMGA1, LPIN1, HAPLN3, FAM171A1, BCL141A, FOXC1, and ANKRD11), and one underexpressed gene (ANX9) are involved in general metabolism. Based on this biochemical peculiarity and the overexpression of BCL11A and FOXC1 (involved in tumor growth and metastasis, respectively), we validated by quantitative polymerase chain reaction the expression profiles of seven genes out of the signature. In this report, a new gene signature for TNBC is proposed. To our knowledge, this is the first TNBC signature that describes genes involved in general metabolism. The findings may be pertinent for Mexican patients and require evaluation in other ethnic groups and populations.",
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Santuario-Facio, SK, Cardona-Huerta, S, Perez-Paramo, YX, Trevino, V, Hernandez-Cabrera, F, Rojas-Martinez, A, Uscanga-Perales, G, Martinez-Rodriguez, JL, Martinez-Jacobo, L, Padilla-Rivas, G, Muñoz-Maldonado, G, Gonzalez-Guerrero, JF, Valero-Gomez, J, Vazquez-Guerrero, AL, Martinez-Rodriguez, HG, Barboza-Quintana, A, Barboza-Quintana, O, Garza-Guajardo, R & Ortiz-Lopez, R 2017, 'A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy', Molecular Medicine, vol. 23, pp. 101-111. https://doi.org/10.2119/molmed.2016.00257

A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy. / Santuario-Facio, Sandra K.; Cardona-Huerta, Servando; Perez-Paramo, Yadira X.; Trevino, Victor; Hernandez-Cabrera, Francisco; Rojas-Martinez, Augusto; Uscanga-Perales, Grecia; Martinez-Rodriguez, Jorge L.; Martinez-Jacobo, Lizeth; Padilla-Rivas, Gerardo; Muñoz-Maldonado, Gerardo; Gonzalez-Guerrero, Juan Francisco; Valero-Gomez, Javier; Vazquez-Guerrero, Ana L.; Martinez-Rodriguez, Herminia G.; Barboza-Quintana, Alvaro; Barboza-Quintana, Oralia; Garza-Guajardo, Raquel; Ortiz-Lopez, Rocio.

In: Molecular Medicine, Vol. 23, 06.2017, p. 101-111.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy

AU - Santuario-Facio, Sandra K.

AU - Cardona-Huerta, Servando

AU - Perez-Paramo, Yadira X.

AU - Trevino, Victor

AU - Hernandez-Cabrera, Francisco

AU - Rojas-Martinez, Augusto

AU - Uscanga-Perales, Grecia

AU - Martinez-Rodriguez, Jorge L.

AU - Martinez-Jacobo, Lizeth

AU - Padilla-Rivas, Gerardo

AU - Muñoz-Maldonado, Gerardo

AU - Gonzalez-Guerrero, Juan Francisco

AU - Valero-Gomez, Javier

AU - Vazquez-Guerrero, Ana L.

AU - Martinez-Rodriguez, Herminia G.

AU - Barboza-Quintana, Alvaro

AU - Barboza-Quintana, Oralia

AU - Garza-Guajardo, Raquel

AU - Ortiz-Lopez, Rocio

PY - 2017/6

Y1 - 2017/6

N2 - Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer tumors. Comparisons between TNBC and non-triple-negative breast cancer (nTNBC) may help to differentiate key components involved in TNBC neoplasms. The purpose of the study was to analyze the expression profile of TNBC versus nTNBC tumors in a homogeneous population from northeastern Mexico. A prospective study of 50 patients (25 TNBC and 25 nTNBC) was conducted. Clinic parameters were equally distributed for TNBC and nTNBC: age at diagnosis (51 versus 47 years, p = 0.1), glucose level (107 mg/dl versus 104 mg/dl, p = 0.64), and body mass index (28 versus 29, p = 0.14). Core biopsies were collected for histopathological diagnosis and gene expression analysis. Total RNA was isolated and expression profiling was performed. Forty genes showed differential expression pattern in TNBC tumors. Among these, nine overexpressed genes (PRKX/PRKY, UGT8, HMGA1, LPIN1, HAPLN3, FAM171A1, BCL141A, FOXC1, and ANKRD11), and one underexpressed gene (ANX9) are involved in general metabolism. Based on this biochemical peculiarity and the overexpression of BCL11A and FOXC1 (involved in tumor growth and metastasis, respectively), we validated by quantitative polymerase chain reaction the expression profiles of seven genes out of the signature. In this report, a new gene signature for TNBC is proposed. To our knowledge, this is the first TNBC signature that describes genes involved in general metabolism. The findings may be pertinent for Mexican patients and require evaluation in other ethnic groups and populations.

AB - Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer tumors. Comparisons between TNBC and non-triple-negative breast cancer (nTNBC) may help to differentiate key components involved in TNBC neoplasms. The purpose of the study was to analyze the expression profile of TNBC versus nTNBC tumors in a homogeneous population from northeastern Mexico. A prospective study of 50 patients (25 TNBC and 25 nTNBC) was conducted. Clinic parameters were equally distributed for TNBC and nTNBC: age at diagnosis (51 versus 47 years, p = 0.1), glucose level (107 mg/dl versus 104 mg/dl, p = 0.64), and body mass index (28 versus 29, p = 0.14). Core biopsies were collected for histopathological diagnosis and gene expression analysis. Total RNA was isolated and expression profiling was performed. Forty genes showed differential expression pattern in TNBC tumors. Among these, nine overexpressed genes (PRKX/PRKY, UGT8, HMGA1, LPIN1, HAPLN3, FAM171A1, BCL141A, FOXC1, and ANKRD11), and one underexpressed gene (ANX9) are involved in general metabolism. Based on this biochemical peculiarity and the overexpression of BCL11A and FOXC1 (involved in tumor growth and metastasis, respectively), we validated by quantitative polymerase chain reaction the expression profiles of seven genes out of the signature. In this report, a new gene signature for TNBC is proposed. To our knowledge, this is the first TNBC signature that describes genes involved in general metabolism. The findings may be pertinent for Mexican patients and require evaluation in other ethnic groups and populations.

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Santuario-Facio SK, Cardona-Huerta S, Perez-Paramo YX, Trevino V, Hernandez-Cabrera F, Rojas-Martinez A et al. A new gene expression signature for Triple-Negative breast cancer using frozen fresh tissue before neoadjuvant chemotherapy. Molecular Medicine. 2017 Jun;23:101-111. https://doi.org/10.2119/molmed.2016.00257