A chemical approach to manipulate the algal growth, lipid content and high-value alpha-linolenic acid for biodiesel production

Ali Parsaeimehr, Elena I. Mancera-Andrade, Felipe Robledo-Padilla, Hafiz M.N. Iqbal, Roberto Parra-Saldivar

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Microalgal cultivation systems for energy production are not yet economically viable due to the low yield of lipids and biomass. In this study, we propose a chemical approach, using exogenous bioactive molecules for improving algal growth, lipid content and the production of high-value alpha-linolenic acid (ALA), using Chlorella protothecoides as a model. Based on our findings, among the cytokinin and auxin classes, Kinetin increased the biosynthesis of ALA by 26.5% at 1 ppm concentration, and indole-3-butyric acid at 2 ppm improved the biomass and lipid productivity up to 29.15 ± 2.02 g m− 2 day− 1, and 6.69 ± 0.56 g m− 2 day− 1, respectively. Methyl jasmonate as a stress-related phytohormone also showed a positive stimulatory effect on growth of C. protothecoides, and the biomass productivity was increased up to 25.26 ± 1.48 g m− 2 day− 1 at the concentration of 5 ppm (lipid content: 219.66 ± 13.20 mg g− 1 dw; lipid productivity: 5.61 ± 0.25 g m− 2 day− 1; ALA: 0.81 ± 0.16 g m− 2 day− 1). A difference in the composition of fatty acids, due to the type and concentrations of stress-related phytohormones was observed, and under the influence of stress-related phytohormones, the biosynthesis of saturated fatty acids increased, and polyunsaturated fatty acids decreased in C. protothecoides. Butylated hydroxyanisole, at a concentration of 0.05 ppm, increased the ALA content by 60.5%. Our study shows that the proposed bioactive molecules could increase the biomass productivity of C. protothecoides at a lower cost compared to the use of acetate as a carbon source.
Original languageEnglish
Pages (from-to)312-322
Number of pages11
JournalAlgal Research
Volume26
DOIs
Publication statusPublished - 1 Sep 2017

Fingerprint

alpha-linolenic acid
biodiesel
lipid content
plant hormones
biomass
lipids
biosynthesis
butylated hydroxyanisole
Chlorella
methyl jasmonate
indole butyric acid
kinetin
cytokinins
saturated fatty acids
polyunsaturated fatty acids
auxins
fatty acid composition
acetates
carbon
energy

Cite this

Parsaeimehr, Ali ; Mancera-Andrade, Elena I. ; Robledo-Padilla, Felipe ; Iqbal, Hafiz M.N. ; Parra-Saldivar, Roberto. / A chemical approach to manipulate the algal growth, lipid content and high-value alpha-linolenic acid for biodiesel production. In: Algal Research. 2017 ; Vol. 26. pp. 312-322.
@article{004421be85324fd4a6b85f4ab6c3f6cf,
title = "A chemical approach to manipulate the algal growth, lipid content and high-value alpha-linolenic acid for biodiesel production",
abstract = "Microalgal cultivation systems for energy production are not yet economically viable due to the low yield of lipids and biomass. In this study, we propose a chemical approach, using exogenous bioactive molecules for improving algal growth, lipid content and the production of high-value alpha-linolenic acid (ALA), using Chlorella protothecoides as a model. Based on our findings, among the cytokinin and auxin classes, Kinetin increased the biosynthesis of ALA by 26.5{\%} at 1 ppm concentration, and indole-3-butyric acid at 2 ppm improved the biomass and lipid productivity up to 29.15 ± 2.02 g m− 2 day− 1, and 6.69 ± 0.56 g m− 2 day− 1, respectively. Methyl jasmonate as a stress-related phytohormone also showed a positive stimulatory effect on growth of C. protothecoides, and the biomass productivity was increased up to 25.26 ± 1.48 g m− 2 day− 1 at the concentration of 5 ppm (lipid content: 219.66 ± 13.20 mg g− 1 dw; lipid productivity: 5.61 ± 0.25 g m− 2 day− 1; ALA: 0.81 ± 0.16 g m− 2 day− 1). A difference in the composition of fatty acids, due to the type and concentrations of stress-related phytohormones was observed, and under the influence of stress-related phytohormones, the biosynthesis of saturated fatty acids increased, and polyunsaturated fatty acids decreased in C. protothecoides. Butylated hydroxyanisole, at a concentration of 0.05 ppm, increased the ALA content by 60.5{\%}. Our study shows that the proposed bioactive molecules could increase the biomass productivity of C. protothecoides at a lower cost compared to the use of acetate as a carbon source.",
author = "Ali Parsaeimehr and Mancera-Andrade, {Elena I.} and Felipe Robledo-Padilla and Iqbal, {Hafiz M.N.} and Roberto Parra-Saldivar",
year = "2017",
month = "9",
day = "1",
doi = "10.1016/j.algal.2017.08.016",
language = "English",
volume = "26",
pages = "312--322",
journal = "Algal Research",
issn = "2211-9264",
publisher = "Elsevier BV",

}

A chemical approach to manipulate the algal growth, lipid content and high-value alpha-linolenic acid for biodiesel production. / Parsaeimehr, Ali; Mancera-Andrade, Elena I.; Robledo-Padilla, Felipe; Iqbal, Hafiz M.N.; Parra-Saldivar, Roberto.

In: Algal Research, Vol. 26, 01.09.2017, p. 312-322.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A chemical approach to manipulate the algal growth, lipid content and high-value alpha-linolenic acid for biodiesel production

AU - Parsaeimehr, Ali

AU - Mancera-Andrade, Elena I.

AU - Robledo-Padilla, Felipe

AU - Iqbal, Hafiz M.N.

AU - Parra-Saldivar, Roberto

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Microalgal cultivation systems for energy production are not yet economically viable due to the low yield of lipids and biomass. In this study, we propose a chemical approach, using exogenous bioactive molecules for improving algal growth, lipid content and the production of high-value alpha-linolenic acid (ALA), using Chlorella protothecoides as a model. Based on our findings, among the cytokinin and auxin classes, Kinetin increased the biosynthesis of ALA by 26.5% at 1 ppm concentration, and indole-3-butyric acid at 2 ppm improved the biomass and lipid productivity up to 29.15 ± 2.02 g m− 2 day− 1, and 6.69 ± 0.56 g m− 2 day− 1, respectively. Methyl jasmonate as a stress-related phytohormone also showed a positive stimulatory effect on growth of C. protothecoides, and the biomass productivity was increased up to 25.26 ± 1.48 g m− 2 day− 1 at the concentration of 5 ppm (lipid content: 219.66 ± 13.20 mg g− 1 dw; lipid productivity: 5.61 ± 0.25 g m− 2 day− 1; ALA: 0.81 ± 0.16 g m− 2 day− 1). A difference in the composition of fatty acids, due to the type and concentrations of stress-related phytohormones was observed, and under the influence of stress-related phytohormones, the biosynthesis of saturated fatty acids increased, and polyunsaturated fatty acids decreased in C. protothecoides. Butylated hydroxyanisole, at a concentration of 0.05 ppm, increased the ALA content by 60.5%. Our study shows that the proposed bioactive molecules could increase the biomass productivity of C. protothecoides at a lower cost compared to the use of acetate as a carbon source.

AB - Microalgal cultivation systems for energy production are not yet economically viable due to the low yield of lipids and biomass. In this study, we propose a chemical approach, using exogenous bioactive molecules for improving algal growth, lipid content and the production of high-value alpha-linolenic acid (ALA), using Chlorella protothecoides as a model. Based on our findings, among the cytokinin and auxin classes, Kinetin increased the biosynthesis of ALA by 26.5% at 1 ppm concentration, and indole-3-butyric acid at 2 ppm improved the biomass and lipid productivity up to 29.15 ± 2.02 g m− 2 day− 1, and 6.69 ± 0.56 g m− 2 day− 1, respectively. Methyl jasmonate as a stress-related phytohormone also showed a positive stimulatory effect on growth of C. protothecoides, and the biomass productivity was increased up to 25.26 ± 1.48 g m− 2 day− 1 at the concentration of 5 ppm (lipid content: 219.66 ± 13.20 mg g− 1 dw; lipid productivity: 5.61 ± 0.25 g m− 2 day− 1; ALA: 0.81 ± 0.16 g m− 2 day− 1). A difference in the composition of fatty acids, due to the type and concentrations of stress-related phytohormones was observed, and under the influence of stress-related phytohormones, the biosynthesis of saturated fatty acids increased, and polyunsaturated fatty acids decreased in C. protothecoides. Butylated hydroxyanisole, at a concentration of 0.05 ppm, increased the ALA content by 60.5%. Our study shows that the proposed bioactive molecules could increase the biomass productivity of C. protothecoides at a lower cost compared to the use of acetate as a carbon source.

UR - http://www.mendeley.com/research/chemical-approach-manipulate-algal-growth-lipid-content-highvalue-alphalinolenic-acid-biodiesel-prod

U2 - 10.1016/j.algal.2017.08.016

DO - 10.1016/j.algal.2017.08.016

M3 - Article

VL - 26

SP - 312

EP - 322

JO - Algal Research

JF - Algal Research

SN - 2211-9264

ER -